Genome Property Definition Page

AccessionGenProp0029
Namenitrogen fixation
TypeMETAPATH
DescriptionNitrogen fixation is the conversion of atmospheric nitrogen, N2 (with its difficult-to-cleave triple bond) into usable forms such as NH3. Conditions should be anaerobic or microaerophilic. The characteristic enzyme is nitrogenase, but many other proteins are involved. Nitrogenase has an iron protein and MoFe, VFe, or FeFe alpha/beta heterotetramer. Expected along with nitrogenase are several accessory proteins, the transcriptional regulator NifA, a ferredoxin or flavodoxin, downstream genes for nitrogen assimilation (NtrC, GlnA), genes for protection from oxygen (e.g. leghemoglobin in root nodule bacteria), etc.
JCVI RoleNitrogen fixation
Parent PropertyGenProp0202: nitrogen metabolism
GenProp0603: lifestyle
GenProp0202: nitrogen metabolism
GenProp0603: lifestyle
GenProp0202: nitrogen metabolism
GenProp0603: lifestyle
Literature References
[ 2 ]Rubio LM, Ludden PW  Maturation of nitrogenase: a biochemical puzzle.  J Bacteriol. 2005 Jan;187(2):405-14.  PMID 15629911
[ 2 ]Rubio LM, Ludden PW  Maturation of nitrogenase: a biochemical puzzle.  J Bacteriol. 2005 Jan;187(2):405-14.  PMID 15629911
[ 2 ]Rubio LM, Ludden PW  Maturation of nitrogenase: a biochemical puzzle.  J Bacteriol. 2005 Jan;187(2):405-14.  PMID 15629911
Web ReferencesKEGG: Nitrogen Metabolism
KEGG: Nitrogen Metabolism
KEGG: Nitrogen Metabolism
Gene Ontology TermGO:0006808: regulation of nitrogen utilization (biological_process)
GO:0006808: regulation of nitrogen utilization (biological_process)
GO:0006808: regulation of nitrogen utilization (biological_process)
GO:0009399: nitrogen fixation (biological_process)
GO:0009399: nitrogen fixation (biological_process)
GO:0009399: nitrogen fixation (biological_process)
GO:0009108: coenzyme biosynthetic process (biological_process)
GO:0009108: coenzyme biosynthetic process (biological_process)
GO:0009108: coenzyme biosynthetic process (biological_process)
GO:0016612: molybdenum-iron nitrogenase complex (cellular_component)
GO:0016612: molybdenum-iron nitrogenase complex (cellular_component)
GO:0016612: molybdenum-iron nitrogenase complex (cellular_component)
GO:0006808: regulation of nitrogen utilization (biological_process)
GO:0006808: regulation of nitrogen utilization (biological_process)
GO:0006808: regulation of nitrogen utilization (biological_process)
GO:0009108: coenzyme biosynthetic process (biological_process)
GO:0009108: coenzyme biosynthetic process (biological_process)
GO:0009108: coenzyme biosynthetic process (biological_process)
GO:0009399: nitrogen fixation (biological_process)
GO:0009399: nitrogen fixation (biological_process)
GO:0009399: nitrogen fixation (biological_process)
GO:0016612: molybdenum-iron nitrogenase complex (cellular_component)
GO:0016612: molybdenum-iron nitrogenase complex (cellular_component)
GO:0016612: molybdenum-iron nitrogenase complex (cellular_component)
GO:0006808: regulation of nitrogen utilization (biological_process)
GO:0006808: regulation of nitrogen utilization (biological_process)
GO:0006808: regulation of nitrogen utilization (biological_process)
GO:0009108: coenzyme biosynthetic process (biological_process)
GO:0009108: coenzyme biosynthetic process (biological_process)
GO:0009108: coenzyme biosynthetic process (biological_process)
GO:0009399: nitrogen fixation (biological_process)
GO:0009399: nitrogen fixation (biological_process)
GO:0009399: nitrogen fixation (biological_process)
GO:0016612: molybdenum-iron nitrogenase complex (cellular_component)
GO:0016612: molybdenum-iron nitrogenase complex (cellular_component)
GO:0016612: molybdenum-iron nitrogenase complex (cellular_component)

Components
Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
nifX neighbor TIGR02935/DUF269DUF269NOTIGR02935 (HMM): probable nitrogen fixation protein
TIGR02935 (HMM): probable nitrogen fixation protein
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nifX neighbor TIGR02935/DUF269DUF269NOTIGR02935 (HMM): probable nitrogen fixation protein
TIGR02935 (HMM): probable nitrogen fixation protein
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nifX neighbor TIGR02935/DUF269DUF269NOTIGR02935 (HMM): probable nitrogen fixation protein
TIGR02935 (HMM): probable nitrogen fixation protein
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogenase component I (dinitrogenase)comp_IYESGenProp0633 (GENPROP): nitrogenase, Mo-containing
GenProp0631 (GENPROP): nitrogenase, Fe-only
GenProp0632 (GENPROP): nitrogenase, V-containing
GenProp0633 (GENPROP): nitrogenase, Mo-containing
GenProp0631 (GENPROP): nitrogenase, Fe-only
GenProp0632 (GENPROP): nitrogenase, V-containing
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogenase component I (dinitrogenase)comp_IYESGenProp0633 (GENPROP): nitrogenase, Mo-containing
GenProp0631 (GENPROP): nitrogenase, Fe-only
GenProp0632 (GENPROP): nitrogenase, V-containing
GenProp0633 (GENPROP): nitrogenase, Mo-containing
GenProp0631 (GENPROP): nitrogenase, Fe-only
GenProp0632 (GENPROP): nitrogenase, V-containing
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogenase component I (dinitrogenase)comp_IYESGenProp0633 (GENPROP): nitrogenase, Mo-containing
GenProp0631 (GENPROP): nitrogenase, Fe-only
GenProp0632 (GENPROP): nitrogenase, V-containing
GenProp0633 (GENPROP): nitrogenase, Mo-containing
GenProp0631 (GENPROP): nitrogenase, Fe-only
GenProp0632 (GENPROP): nitrogenase, V-containing
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogenase component II (dinitrogenase reductase)comp_IIYESTIGR01287 (HMM): nitrogenase iron protein
TIGR01287 (HMM): nitrogenase iron protein
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogenase component II (dinitrogenase reductase)comp_IIYESTIGR01287 (HMM): nitrogenase iron protein
TIGR01287 (HMM): nitrogenase iron protein
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogenase component II (dinitrogenase reductase)comp_IIYESTIGR01287 (HMM): nitrogenase iron protein
TIGR01287 (HMM): nitrogenase iron protein
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
ADP-ribosyl-[dinitrogen reductase] hydrolasedraGNOTIGR02662 (HMM): ADP-ribosyl-[dinitrogen reductase] hydrolase
TIGR02662 (HMM): ADP-ribosyl-[dinitrogen reductase] hydrolase
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
ADP-ribosyl-[dinitrogen reductase] hydrolasedraGNOTIGR02662 (HMM): ADP-ribosyl-[dinitrogen reductase] hydrolase
TIGR02662 (HMM): ADP-ribosyl-[dinitrogen reductase] hydrolase
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
ADP-ribosyl-[dinitrogen reductase] hydrolasedraGNOTIGR02662 (HMM): ADP-ribosyl-[dinitrogen reductase] hydrolase
TIGR02662 (HMM): ADP-ribosyl-[dinitrogen reductase] hydrolase
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferasedraTNOPF07357 (HMM): NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferase
PF07357 (HMM): NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferase
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferasedraTNOPF07357 (HMM): NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferase
PF07357 (HMM): NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferase
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferasedraTNOPF07357 (HMM): NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferase
PF07357 (HMM): NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferase
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
transcriptional regulatornifANOTIGR01817 (HMM): Nif-specific regulatory protein
TIGR01817 (HMM): Nif-specific regulatory protein
GO:0006808: regulation of nitrogen utilization
GO:0006808: regulation of nitrogen utilization
transcriptional regulatornifANOTIGR01817 (HMM): Nif-specific regulatory protein
TIGR01817 (HMM): Nif-specific regulatory protein
GO:0006808: regulation of nitrogen utilization
GO:0006808: regulation of nitrogen utilization
transcriptional regulatornifANOTIGR01817 (HMM): Nif-specific regulatory protein
TIGR01817 (HMM): Nif-specific regulatory protein
GO:0006808: regulation of nitrogen utilization
GO:0006808: regulation of nitrogen utilization
nitrogenase iron-molybdenum cofactor biosynthesis proteinnifBYESTIGR01290 (HMM): nitrogenase cofactor biosynthesis protein NifB
TIGR01290 (HMM): nitrogenase cofactor biosynthesis protein NifB
GO:0009108: coenzyme biosynthetic process
GO:0009108: coenzyme biosynthetic process
nitrogenase iron-molybdenum cofactor biosynthesis proteinnifBYESTIGR01290 (HMM): nitrogenase cofactor biosynthesis protein NifB
TIGR01290 (HMM): nitrogenase cofactor biosynthesis protein NifB
GO:0009108: coenzyme biosynthetic process
GO:0009108: coenzyme biosynthetic process
nitrogenase iron-molybdenum cofactor biosynthesis proteinnifBYESTIGR01290 (HMM): nitrogenase cofactor biosynthesis protein NifB
TIGR01290 (HMM): nitrogenase cofactor biosynthesis protein NifB
GO:0009108: coenzyme biosynthetic process
GO:0009108: coenzyme biosynthetic process
nitrogenase iron-molybdenum cofactor biosynthesis proteinnifEYESTIGR01283 (HMM): nitrogenase MoFe cofactor biosynthesis protein NifE
TIGR01283 (HMM): nitrogenase MoFe cofactor biosynthesis protein NifE
GO:0009108: coenzyme biosynthetic process
GO:0009108: coenzyme biosynthetic process
nitrogenase iron-molybdenum cofactor biosynthesis proteinnifEYESTIGR01283 (HMM): nitrogenase MoFe cofactor biosynthesis protein NifE
TIGR01283 (HMM): nitrogenase MoFe cofactor biosynthesis protein NifE
GO:0009108: coenzyme biosynthetic process
GO:0009108: coenzyme biosynthetic process
nitrogenase iron-molybdenum cofactor biosynthesis proteinnifEYESTIGR01283 (HMM): nitrogenase MoFe cofactor biosynthesis protein NifE
TIGR01283 (HMM): nitrogenase MoFe cofactor biosynthesis protein NifE
GO:0009108: coenzyme biosynthetic process
GO:0009108: coenzyme biosynthetic process
nitrogen fixation positive activator proteinnifLNO
nitrogen fixation positive activator proteinnifLNO
nitrogen fixation positive activator proteinnifLNO
nitrogen fixation protein NifMnifMNOTIGR02933 (HMM): nitrogen fixation protein NifM
TIGR02933 (HMM): nitrogen fixation protein NifM
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogen fixation protein NifMnifMNOTIGR02933 (HMM): nitrogen fixation protein NifM
TIGR02933 (HMM): nitrogen fixation protein NifM
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogen fixation protein NifMnifMNOTIGR02933 (HMM): nitrogen fixation protein NifM
TIGR02933 (HMM): nitrogen fixation protein NifM
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogenase molybdenum-iron protein, NifN subunitnifNYESTIGR01285 (HMM): nitrogenase molybdenum-iron cofactor biosynthesis protein NifN
TIGR01285 (HMM): nitrogenase molybdenum-iron cofactor biosynthesis protein NifN
GO:0016612: molybdenum-iron nitrogenase complex
GO:0016612: molybdenum-iron nitrogenase complex
nitrogenase molybdenum-iron protein, NifN subunitnifNYESTIGR01285 (HMM): nitrogenase molybdenum-iron cofactor biosynthesis protein NifN
TIGR01285 (HMM): nitrogenase molybdenum-iron cofactor biosynthesis protein NifN
GO:0016612: molybdenum-iron nitrogenase complex
GO:0016612: molybdenum-iron nitrogenase complex
nitrogenase molybdenum-iron protein, NifN subunitnifNYESTIGR01285 (HMM): nitrogenase molybdenum-iron cofactor biosynthesis protein NifN
TIGR01285 (HMM): nitrogenase molybdenum-iron cofactor biosynthesis protein NifN
GO:0016612: molybdenum-iron nitrogenase complex
GO:0016612: molybdenum-iron nitrogenase complex
nitrogen fixation protein NifQnifQNOPF04891 (HMM): NifQ
PF04891 (HMM): NifQ
nitrogen fixation protein NifQnifQNOPF04891 (HMM): NifQ
PF04891 (HMM): NifQ
nitrogen fixation protein NifQnifQNOPF04891 (HMM): NifQ
PF04891 (HMM): NifQ
probable nitrogen fixation protein NifTnifTNOTIGR02934 (HMM): probable nitrogen fixation protein FixT
TIGR02934 (HMM): probable nitrogen fixation protein FixT
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
probable nitrogen fixation protein NifTnifTNOTIGR02934 (HMM): probable nitrogen fixation protein FixT
TIGR02934 (HMM): probable nitrogen fixation protein FixT
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
probable nitrogen fixation protein NifTnifTNOTIGR02934 (HMM): probable nitrogen fixation protein FixT
TIGR02934 (HMM): probable nitrogen fixation protein FixT
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
homocitrate synthasenifVNOTIGR02660 (HMM): homocitrate synthase
TIGR02660 (HMM): homocitrate synthase
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
homocitrate synthasenifVNOTIGR02660 (HMM): homocitrate synthase
TIGR02660 (HMM): homocitrate synthase
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
homocitrate synthasenifVNOTIGR02660 (HMM): homocitrate synthase
TIGR02660 (HMM): homocitrate synthase
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogen fixation proteinnifWNOPF03206 (HMM): nitrogen fixation protein NifW
PF03206 (HMM): nitrogen fixation protein NifW
nitrogen fixation proteinnifWNOPF03206 (HMM): nitrogen fixation protein NifW
PF03206 (HMM): nitrogen fixation protein NifW
nitrogen fixation proteinnifWNOPF03206 (HMM): nitrogen fixation protein NifW
PF03206 (HMM): nitrogen fixation protein NifW
nitrogenase molybdenum-iron cofactor biosynthesis proteinnifXNOTIGR02663 (HMM): nitrogen fixation protein NifX
TIGR02663 (HMM): nitrogen fixation protein NifX
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogenase molybdenum-iron cofactor biosynthesis proteinnifXNOTIGR02663 (HMM): nitrogen fixation protein NifX
TIGR02663 (HMM): nitrogen fixation protein NifX
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nitrogenase molybdenum-iron cofactor biosynthesis proteinnifXNOTIGR02663 (HMM): nitrogen fixation protein NifX
TIGR02663 (HMM): nitrogen fixation protein NifX
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
iron-sulfur cofactor synthesis proteinnifZNOPF04319 (HMM): NifZ domain
PF04319 (HMM): NifZ domain
iron-sulfur cofactor synthesis proteinnifZNOPF04319 (HMM): NifZ domain
PF04319 (HMM): NifZ domain
iron-sulfur cofactor synthesis proteinnifZNOPF04319 (HMM): NifZ domain
PF04319 (HMM): NifZ domain
nif-specific ferredoxinnif_fdxNOTIGR02936 (HMM): ferredoxin III, nif-specific
TIGR02936 (HMM): ferredoxin III, nif-specific
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nif-specific ferredoxinnif_fdxNOTIGR02936 (HMM): ferredoxin III, nif-specific
TIGR02936 (HMM): ferredoxin III, nif-specific
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation
nif-specific ferredoxinnif_fdxNOTIGR02936 (HMM): ferredoxin III, nif-specific
TIGR02936 (HMM): ferredoxin III, nif-specific
GO:0009399: nitrogen fixation
GO:0009399: nitrogen fixation

Parent Properties
AccessionName
GenProp0202nitrogen metabolism
GenProp0603lifestyle
GenProp0202nitrogen metabolism
GenProp0603lifestyle
GenProp0202nitrogen metabolism
GenProp0603lifestyle

Child Properties
AccessionName
GenProp0631nitrogenase, Fe-only
GenProp0632nitrogenase, V-containing
GenProp0633nitrogenase, Mo-containing
GenProp0631nitrogenase, Fe-only
GenProp0632nitrogenase, V-containing
GenProp0633nitrogenase, Mo-containing
GenProp0631nitrogenase, Fe-only
GenProp0632nitrogenase, V-containing
GenProp0633nitrogenase, Mo-containing

Sibling Properties
AccessionName
GenProp0200periplasmic nitrate reductase
GenProp0666membrane-associated monooxygenase (methane/ammonia)
GenProp0682formate-dependent nitrite reductase, seven-gene type
GenProp0683cytochrome c nitrite reductase NrfHA
GenProp0814urea utilization
GenProp0899nitrile hydratase
GenProp0011methanogenesis
GenProp0013oxygen requirement
GenProp0031methane utilization
GenProp0044temperature environment
GenProp0153biofilm participation
GenProp0549pathogen
GenProp0554mutualist
GenProp0558obligate intracellular growth
GenProp0559facultative intracellular growth
GenProp0602extremophile
GenProp0608chemoautotroph
GenProp0609phototroph