Genome Property Definition Page

DescriptionUrease is a multi-subunit, nickel-containing enzyme required for the hydrolysis of urea to carbon dioxide and ammonia [1]. Its three structural genes are located in an unidirectionally transcribed gene cluster consisting either as separate alpha, beta, and gamma subunits, or as in Helicobacter pylori, a fusion of beta and gamma subunits [2]. Associated with these structural subunits are accessory factors functioning to assemble and incorporate its nickel-containing metallocenter [3]. This property predicts a urease system to be present if both its three structural subunits and four accessory proteins are all found. In Bacillus subtilis, the accessory proteins are not found by homology but urease function has been shown experimentally. This finding raises the question of what alternative mechanism can enable the assembly of functional urease.
JCVI RoleNitrogen metabolism
Parent PropertyGenProp0074: virulence
GenProp0814: urea utilization
Literature References
[ 1 ]Mobley HL, Hausinger RP.  Microbial ureases: significance, regulation, and molecular characterization.  Microbiol Rev. 1989 Mar;53(1):85-108. Review.  PMID 2651866
[ 2 ]Ha NC, Oh ST, Sung JY, Cha KA, Lee MH, Oh BH.  Supramolecular assembly and acid resistance of Helicobacter pylori urease.  Nat Struct Biol. 2001 Jun;8(6):505-9.  PMID 11373617
[ 3 ]Moncrief MB, Hausinger RP.  Characterization of UreG, identification of a UreD-UreF-UreG complex, and evidence suggesting that a nucleotide-binding site in UreG is required for in vivo metallocenter assembly of Klebsiella aerogenes urease.  J Bacteriol. 1997 Jul;179(13):4081-6.  PMID 9209019
Gene Ontology TermGO:0019627: urea metabolic process (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
nickel transporter (UreH)Ni_transNOTIGR00802 (HMM): transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family
urease, gamma subunitureAYESTIGR00193 (HMM): urease, gamma subunitGO:0019627: urea metabolic process
urease, beta subunitureBYESTIGR00192 (HMM): urease, beta subunitGO:0019627: urea metabolic process
urease, alpha subunitureCYESTIGR01792 (HMM): urease, alpha subunit
alpha-subunit, N-terminal domainureC-NYESPF00449 (HMM): urease alpha-subunit, N-terminal domainGO:0019627: urea metabolic process
urease accessory protein UreDureDYESPF01774 (HMM): urease accessory protein UreDGO:0019627: urea metabolic process
urease accessory protein UreEureEYESPF02814 (HMM): urease accessory protein UreE, N-terminal domainGO:0019627: urea metabolic process
urease accessory protein UreFureFYESPF01730 (HMM): urease accessory protein UreFGO:0019627: urea metabolic process
urease accessory protein UreGureGYESTIGR00101 (HMM): urease accessory protein UreGGO:0019627: urea metabolic process

Parent Properties
GenProp0814urea utilization

Sibling Properties
GenProp0032pathogen of plants
GenProp0049spore formation
GenProp0052type III secretion
GenProp0056type IV pilus
GenProp0154intrinsic pathogen of animals
GenProp0648phage shock protein regulon
GenProp0707botulinum toxin system
GenProp0849quorum-sensing, Bacillus PlcR-PapR system
GenProp0481urea carboxylase/allophanate hydrolase pathway
GenProp0743urea ABC transporter UrtABCDE