| Accession | GenProp0051 |
| Name | urease |
| Type | SYSTEM |
| Description | Urease is a multi-subunit, nickel-containing enzyme required for the hydrolysis of urea to carbon dioxide and ammonia [1]. Its three structural genes are located in an unidirectionally transcribed gene cluster consisting either as separate alpha, beta, and gamma subunits, or as in Helicobacter pylori, a fusion of beta and gamma subunits [2]. Associated with these structural subunits are accessory factors functioning to assemble and incorporate its nickel-containing metallocenter [3]. This property predicts a urease system to be present if both its three structural subunits and four accessory proteins are all found. In Bacillus subtilis, the accessory proteins are not found by homology but urease function has been shown experimentally. This finding raises the question of what alternative mechanism can enable the assembly of functional urease. |
| JCVI Role | Nitrogen metabolism |
| Parent Property | GenProp0074: virulence
GenProp0814: urea utilization
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| Literature References | [ 1 ]Mobley HL, Hausinger RP. Microbial ureases: significance, regulation, and molecular characterization. Microbiol Rev. 1989 Mar;53(1):85-108. Review. PMID 2651866
[ 2 ]Ha NC, Oh ST, Sung JY, Cha KA, Lee MH, Oh BH. Supramolecular assembly and acid resistance of Helicobacter pylori urease. Nat Struct Biol. 2001 Jun;8(6):505-9. PMID 11373617
[ 3 ]Moncrief MB, Hausinger RP. Characterization of UreG, identification of a UreD-UreF-UreG complex, and evidence suggesting that a nucleotide-binding site in UreG is required for in vivo metallocenter assembly of Klebsiella aerogenes urease. J Bacteriol. 1997 Jul;179(13):4081-6. PMID 9209019
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| Gene Ontology Term | GO:0019627: urea metabolic process (biological_process)
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| Step Name | Step Num | Required | Evidence (Method) | Evidence Go Terms |
|---|
| nickel transporter (UreH) | Ni_trans | NO | TIGR00802 (HMM): transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family | |
| urease, gamma subunit | ureA | YES | TIGR00193 (HMM): urease, gamma subunit | GO:0019627: urea metabolic process
|
| urease, beta subunit | ureB | YES | TIGR00192 (HMM): urease, beta subunit | GO:0019627: urea metabolic process
|
| urease, alpha subunit | ureC | YES | TIGR01792 (HMM): urease, alpha subunit | |
| alpha-subunit, N-terminal domain | ureC-N | YES | PF00449 (HMM): urease alpha-subunit, N-terminal domain | GO:0019627: urea metabolic process
|
| urease accessory protein UreD | ureD | YES | PF01774 (HMM): urease accessory protein UreD | GO:0019627: urea metabolic process
|
| urease accessory protein UreE | ureE | YES | PF02814 (HMM): urease accessory protein UreE, N-terminal domain | GO:0019627: urea metabolic process
|
| urease accessory protein UreF | ureF | YES | PF01730 (HMM): urease accessory protein UreF | GO:0019627: urea metabolic process
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| urease accessory protein UreG | ureG | YES | TIGR00101 (HMM): urease accessory protein UreG | GO:0019627: urea metabolic process
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