Genome Property Definition Page

AccessionGenProp0250
Namethiamine: hydroxyethylthiazole from 1-deoxy-D-xylulose-5-phosphate, cysteine, either Tyr or Gly
TypePATHWAY
DescriptionThis property describes de novo synthesis of the thiazole moiety of thiamine, hydroxyethylthiazole (HET). This requires more distinct proteins than that of the pyrimidine moeity, hydromethylpyrimidine (HMP). Phosphorylated forms of HET and HMP are connected by ThiE as thiamine phosphate and converted eventually to the active form thiamine pyrophosphate, TPP. HET biosynthesis requires the small ThiS protein, the C-terminus of which becomes modified transiently by a sulfur atom from cysteine. ThiG is required, along with either ThiH in species where tyrosine is a precursor or ThiO in species where glycine is a precursor. ThiF, although required biosynthetically, is not readily separated from MoeB, a molybdopterin biosynthesis protein, and so is not built into the rule as a required component. Either IscS or NifS must be present, but this requirement also is not included in the rule. The ThiI gene is required, but also serves in formation of a tRNA modified site in species that do not make HET. This property represents and alternative to HET uptake as a means to obtain the thiazole component for thiamine biosynthesis.
JCVI RoleThiamine
Parent PropertyGenProp0184: cofactor biosynthesis
GenProp0261: thiamin pyrophosphate (TPP) de novo biosynthesis
Literature References
[ 1 ]Winkler W, Nahvi A, Breaker RR  Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression.  Nature 2002 Oct 31;419(6910):952-6.  PMID 12410317
[ 2 ]Rodionov DA, Vitreschak AG, Mironov AA, Gelfand MS  Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms.  J Biol Chem 2002 Dec 13;277(50):48949-59. Epub 2002 Oct 09.  PMID 12376536
Gene Ontology TermGO:0009228: thiamine biosynthetic process (biological_process)
GO:0018131: oxazole or thiazole biosynthetic process (biological_process)

Components
Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
ThiH (Tyr) or ThiO (Gly)H_or_OYESTIGR02352 (HMM): glycine oxidase ThiO
TIGR02351 (HMM): thiazole biosynthesis protein ThiH
GO:0009228: thiamine biosynthetic process
GO:0018131: oxazole or thiazole biosynthetic process
GO:0009228: thiamine biosynthetic process
GO:0018131: oxazole or thiazole biosynthetic process
ThiF proteinThiFNOTIGR02354 (HMM): thiamine biosynthesis protein ThiF
TIGR02356 (HMM): thiazole biosynthesis adenylyltransferase ThiF
GO:0009228: thiamine biosynthetic process
GO:0018131: oxazole or thiazole biosynthetic process
GO:0009228: thiamine biosynthetic process
GO:0018131: oxazole or thiazole biosynthetic process
ThiG proteinThiGYESPF05690 (HMM): thiazole biosynthesis protein ThiGGO:0009228: thiamine biosynthetic process
GO:0018131: oxazole or thiazole biosynthetic process
ThiI proteinThiIYESTIGR04271 (HMM): thiazole biosynthesis domainGO:0009228: thiamine biosynthetic process
GO:0018131: oxazole or thiazole biosynthetic process
ThiS proteinThiSYESTIGR01683 (HMM): thiamine biosynthesis protein ThiSGO:0009228: thiamine biosynthetic process
GO:0018131: oxazole or thiazole biosynthetic process

Parent Properties
AccessionName
GenProp0184cofactor biosynthesis
GenProp0261thiamin pyrophosphate (TPP) de novo biosynthesis


Sibling Properties
AccessionName
GenProp0001chorismate biosynthesis via shikimate
GenProp0030glutathione biosynthesis
GenProp0036biotin biosynthesis
GenProp0038tetrahydrofolate biosynthesis from GTP and PABA
GenProp0057NAD(P) biosynthesis from L-aspartate and DHAP
GenProp0112riboflavin, FAD and FMN from GTP and ribulose-5-P
GenProp0124pantothenate biosynthesis from aspartate and 2-oxoisovalerate
GenProp0136ubiquinone biosynthesis from chorismate, aerobic
GenProp0140iron-sulfur cluster assembly systems
GenProp0170coenzyme PQQ biosynthesis
GenProp0171coenzyme A biosynthesis from pantothenate
GenProp0194siroheme biosynthesis from uroporphrinogen-III
GenProp0220uroporhyrinogen III biosynthesis from 5-aminolevulinate
GenProp0221protoporphyrin IX biosynthesis from uroporphyrinogen III
GenProp0222protoheme from protoporphyrin IX
GenProp02555-aminolevulinate biosynthesis
GenProp0261thiamin pyrophosphate (TPP) de novo biosynthesis
GenProp0274coenzyme B12 biosynthesis - overview
GenProp0300photosynthetic cofactor biosynthesis
GenProp0466molybdopterin biosynthesis
GenProp0752ergothioneine biosynthesis
GenProp0753mycothiol biosynthesis
GenProp0759para-aminobenzoic acid (PABA) biosynthesis from chorismate
GenProp0791coenzyme F420 biosynthesis
GenProp07927,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) biosynthesis
GenProp0836menaquinone biosynthesis
GenProp0860tryptophan tryptophylquinone modification of methylamine dehydrogenase
GenProp0862pyridoxal phosphate biosynthesis, PdxA/PdxJ pathway
GenProp0863pyridoxal phosphate biosynthesis, Pdx1/Pdx2 pathway
GenProp0891coenzyme M biosynthesis
GenProp0917mycofactocin system
GenProp0927bacillithiol biosynthesis
GenProp1064ovothiol biosynthesis
GenProp0253thiamine: hydroxymethylpyrimidine pyrophosphate from aminoimidazole ribotide
GenProp0254thiamine: TPP from HMP-PP and HET-P