Genome Property Definition Page

Nameiron-sulfur cluster assembly NIF system
DescriptionIron-sulfur (FeS) cluster assembly systems include the NIF system, which is part of nitrogen fixation clusters in a number of bacteria, as well as the ISC and SUF systems. The latter two make FeS clusters for a wide variety of proteins, while NIF and a related system called CsdA-CsdE may produce FeS clusters for only a small range of target proteins. The defining components of the NIF-type systems described here are the cysteine desulfurase NifS and the FeS cluster assembly scaffold protein NifU. The terms NifS and NifU are applied not only to components of nitrogen fixation systems, but also to tandem gene pairs in other organisms such as Helicobacter pylori. These NifU-like and NifS-like proteins largely fall into separate clades, and are best modeled by separate HMMs, but are included in this Genome Property as components of NIF-type systems. NifS/NifU or NifS-like/NifU-like pairs may produce FeS clusters for a limited range of target proteins, as in Azotobacter vinelandii, or uniquely within the cell, as in Helicobacter pylori.
Parent PropertyGenProp0140: iron-sulfur cluster assembly systems
Literature References
[ 1 ]Yuvaniyama P, Agar JN, Cash VL, Johnson MK, Dean DR  NifS-directed assembly of a transient [2Fe-2S] cluster within the NifU protein.  Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):599-604.  PMID 10639125
[ 2 ]Johnson DC, Dos Santos PC, Dean DR  NifU and NifS are required for the maturation of nitrogenase and cannot replace the function of isc-gene products in Azotobacter vinelandii.  Biochem Soc Trans. 2005 Feb;33(Pt 1):90-3.  PMID 15667274
Gene Ontology TermGO:0016226: iron-sulfur cluster assembly (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
cysteine desulfurase NifSNifSYESTIGR03402 (HMM): cysteine desulfurase NifS
TIGR03403 (HMM): cysteine desulfurase, NifS family
GO:0016226: iron-sulfur cluster assembly
GO:0016226: iron-sulfur cluster assembly
FeS cluster formation protein NifUNifUYESTIGR02000 (HMM): Fe-S cluster assembly protein NifU
TIGR03419 (HMM): FeS cluster assembly scaffold protein NifU
GO:0016226: iron-sulfur cluster assembly
GO:0016226: iron-sulfur cluster assembly

Parent Properties
GenProp0140iron-sulfur cluster assembly systems

Sibling Properties
GenProp0137iron-sulfur cluster assembly SUF system
GenProp0138iron-sulfur cluster assembly iscSUA-hscBA-fdx system
GenProp0739iron-sulfur cluster assembly CsdA-CsdE system