Genome Property Definition Page

AccessionGenProp0752
Nameergothioneine biosynthesis
TypeSYSTEM
DescriptionIn actinobacteria, ergothioneine biosynthesis begins with EgtD, a SAM-dependent enzyme that trimethylates the histidine alpha-amino group to produce hercynine, the histidine-betaine. EgtA gamma-ligates Cys to Glu, as occurs in glutathione biosynthesis. Next, EgtB attaches hercynine to gammaglutamyl cysteine, with sulfur attachment to the histidine-derived ring. EgtC then releases glutamate . Finally, EgtE, a pyridoxal phosphate-dependent enzyme related to selenocysteine lyase, cleaves the hercynylcysteine sulfoxide to leave ergothioneine. Ergothioneine production is known also in cyanobacteria and mushrooms, and is predicted to be more widely distributed.
JCVI RoleConserved
Parent PropertyGenProp0184: cofactor biosynthesis
Literature References
[ 1 ]Seebeck FP  In vitro reconstitution of Mycobacterial ergothioneine biosynthesis.  J Am Chem Soc. 2010 May 19;132(19):6632-3.  PMID 20420449
Gene Ontology TermGO:0052699: ergothioneine biosynthetic process (biological_process)

Components
Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
glutamate--cysteine ligase family proteinegtANOTIGR03444 (HMM): ergothioneine biosynthesis glutamate--cysteine ligase EgtAGO:0052699: ergothioneine biosynthetic process
ergothioneine biosynthesis protein EgtBegtBYESTIGR03440 (HMM): ergothioneine biosynthesis protein EgtBGO:0052699: ergothioneine biosynthetic process
ergothioneine biosynthesis protein EgtCegtCNOTIGR03442 (HMM): ergothioneine biosynthesis protein EgtCGO:0052699: ergothioneine biosynthetic process
ergothioneine biosynthesis methyltransferase EgtDegtDYESTIGR03438 (HMM): dimethylhistidine N-methyltransferaseGO:0052699: ergothioneine biosynthetic process
ergothioneine biosynthesis PLP-binding protein EgtEegtENOTIGR04343 (HMM): ergothioneine biosynthesis PLP-dependent enzyme EgtEGO:0052699: ergothioneine biosynthetic process

Parent Properties
AccessionName
GenProp0184cofactor biosynthesis


Sibling Properties
AccessionName
GenProp0001chorismate biosynthesis via shikimate
GenProp0030glutathione biosynthesis
GenProp0036biotin biosynthesis
GenProp0038tetrahydrofolate biosynthesis from GTP and PABA
GenProp0057NAD(P) biosynthesis from L-aspartate and DHAP
GenProp0112riboflavin, FAD and FMN from GTP and ribulose-5-P
GenProp0124pantothenate biosynthesis from aspartate and 2-oxoisovalerate
GenProp0136ubiquinone biosynthesis from chorismate, aerobic
GenProp0140iron-sulfur cluster assembly systems
GenProp0170coenzyme PQQ biosynthesis
GenProp0171coenzyme A biosynthesis from pantothenate
GenProp0194siroheme biosynthesis from uroporphrinogen-III
GenProp0220uroporhyrinogen III biosynthesis from 5-aminolevulinate
GenProp0221protoporphyrin IX biosynthesis from uroporphyrinogen III
GenProp0222protoheme from protoporphyrin IX
GenProp0250thiamine: hydroxyethylthiazole from 1-deoxy-D-xylulose-5-phosphate, cysteine, either Tyr or Gly
GenProp02555-aminolevulinate biosynthesis
GenProp0261thiamin pyrophosphate (TPP) de novo biosynthesis
GenProp0274coenzyme B12 biosynthesis - overview
GenProp0300photosynthetic cofactor biosynthesis
GenProp0466molybdopterin biosynthesis
GenProp0753mycothiol biosynthesis
GenProp0759para-aminobenzoic acid (PABA) biosynthesis from chorismate
GenProp0791coenzyme F420 biosynthesis
GenProp07927,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) biosynthesis
GenProp0836menaquinone biosynthesis
GenProp0860tryptophan tryptophylquinone modification of methylamine dehydrogenase
GenProp0862pyridoxal phosphate biosynthesis, PdxA/PdxJ pathway
GenProp0863pyridoxal phosphate biosynthesis, Pdx1/Pdx2 pathway
GenProp0891coenzyme M biosynthesis
GenProp0917mycofactocin system
GenProp0927bacillithiol biosynthesis
GenProp1064ovothiol biosynthesis