Genome Property Definition Page

Nameproposed N-acetyl sugar amidation module WbuXYZ
DescriptionThe WbuX protein from E. coli and its homologs in P. aeruginosa (IfnA) and C. jejuni (PseA) have been proposed to function as N-acetyl sugar amidotransferases, forming the positively charged acetamidino moitey on both O-antigens and glycosylated flagellins [1,2]. These WbuX homologs are invariably associated with homologs of the HisH and HisF proteins (WbuYZ) which are proposed to act as a glutaminase and ammonium channel to provide sufficient ammonium ion under limiting conditions [3]. Occasionally the WbuX protein is found in multiple (tandem) copies which may indicate multiple substrates or multiple N-acetyl groups being modified on the same substrate.
Parent PropertyGenProp0795: carbohydrate biosynthesis
Literature References
[ 1 ] King JD, Mulrooney EF, Vinogradov E, Kneidinger B, Mead K, Lam JS   lfnA from Pseudomonas aeruginosa O12 and wbuX from Escherichia coli O145 encode membrane-associated proteins and are required for expression of 2,6-dideoxy-2-acetamidino-L-galactose in lipopolysaccharide O antigen.   J Bacteriol. 2008 Mar;190(5):1671-9. Epub 2007 Dec 21.  PMID 18156256
[ 2 ] McNally DJ, Hui JP, Aubry AJ, Mui KK, Guerry P, Brisson JR, Logan SM, Soo EC   Functional characterization of the flagellar glycosylation locus in Campylobacter jejuni 81-176 using a focused metabolomics approach.   J Biol Chem. 2006 Jul 7;281(27):18489-98. Epub 2006 May 9.  PMID 16684771
[ 3 ] Feng L, Senchenkova SN, Tao J, Shashkov AS, Liu B, Shevelev SD, Reeves PR, Xu J, Knirel YA, Wang L   Structural and genetic characterization of enterohemorrhagic Escherichia coli O145 O antigen and development of an O145 serogroup-specific PCR assay.   J Bacteriol. 2005 Jan;187(2):758-64.  PMID 15629947

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
N-acetyl sugar amidotransferaseWbuXYESTIGR03573 (HMM): N-acetyl sugar amidotransferase
HisH-like glutaminase WbuYWbuYYESTIGR01855 (HMM-CLUST): imidazole glycerol phosphate synthase, glutamine amidotransferase subunit
HisF-like proposed ammonium channel protein WbuZWbuZYESTIGR03572 (HMM): glycosyl amidation-associated protein WbuZ
2918 (RULE_BASE)

Parent Properties
GenProp0795carbohydrate biosynthesis

Sibling Properties
GenProp0183dTDP-L-rhamnose biosynthesis from dTDP-4-dehydro-L-rhamnose
GenProp0264glucosylglycerol biosynthesis
GenProp0297lipopolysaccharide biosynthesis
GenProp0750UDP-N-acetylglucosamine biosynthesis from fructose-6-phosphate
GenProp0766anhydro-N-acetylmuramic acid to N-acetylglucosamine-phosphate
GenProp0793CMP-N-acetylneuraminate biosynthesis from UDP-N-acetylglucosamine
GenProp0796CMP-pseudaminic acid biosynthesis from UDP-N-acetylglucosamine
GenProp0811S-layer homology domain-mediated cell wall binding