Genome Property Definition Page

Namemycofactocin system
DescriptionThis Genome Property describes the proposed mycofactocin system, in which the four absolutely conserved elements are a radical SAM protein, a glycosyltransferase, a small protein with a strongly conserved central motif, and a very small protein with an invariant C-terminal motif GCVY. This last component, the proposed mycofactocin precursor MftA, often is not apparent because of faulty gene calling. Species with this system in addition always have at least a few examples (usually co-clustered) of nicotinoproteins with tightly bound NAD(P) cofactors that cannot be exchanged, requiring an extrinsic mechanism (such as the artifical electron acceptor NDMA) to be able to cycle. Mycofactocin is proposed to undergo modification and become the required electron exchange cofactor.
JCVI RoleOther
Parent PropertyGenProp0184: cofactor biosynthesis
Literature References
[ 1 ]Haft DH  Bioinformatic evidence for a widely distributed, ribosomally produced electron carrier precursor, its maturation proteins, and its nicotinoprotein redox partners.  BMC Genomics. 2011 Jan 11;12:21.  PMID 21223593
[ 2 ]Bystrykh LV, Govorukhina NI, Dijkhuizen L, Duine JA  Tetrazolium-dye-linked alcohol dehydrogenase of the methylotrophic actinomycete Amycolatopsis methanolica is a three-component complex.  Eur J Biochem. 1997 Jul 1;247(1):280-7.  PMID 9249038
Gene Ontology TermGO:0031179: peptide modification (biological_process)
GO:0051188: cofactor biosynthetic process (biological_process)
GO:0051193: regulation of cofactor metabolic process (biological_process)
GO:0055114: oxidation-reduction process (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
oxidoreductase, FAD/FMN-bindingac_AD_4NOTIGR03997 (HMM): mycofactocin system FadH/OYE family oxidoreductase 2
TIGR03996 (HMM): mycofactocin system FadH/OYE family oxidoreductase 1
GO:0055114: oxidation-reduction process
GO:0051188: cofactor biosynthetic process
GO:0031179: peptide modification
dehydrogenase, Rv0697 familyac_aux1NOTIGR03970 (HMM): dehydrogenase, Rv0697 familyGO:0055114: oxidation-reduction process
mycofactocin precursormftAYESTIGR03969 (HMM): mycofactocin precursorGO:0051188: cofactor biosynthetic process
GO:0031179: peptide modification
RPExGFAL proteinmftBYESTIGR03967 (HMM): putative mycofactocin binding protein MftBGO:0051188: cofactor biosynthetic process
GO:0031179: peptide modification
radical SAM mycofactocin maturasemftCSAMYESTIGR03962 (HMM): mycofactocin radical SAM maturaseGO:0051188: cofactor biosynthetic process
GO:0031179: peptide modification
heme/FMN protein,mftDflvYESTIGR03966 (HMM): heme/flavin dehydrogenase, mycofactocin systemGO:0055114: oxidation-reduction process
creatininase family proteinmftEcreYESTIGR03964 (HMM): mycofactocin system creatininase family proteinGO:0051188: cofactor biosynthetic process
GO:0031179: peptide modification
glycosyltransferasemftFglyYESTIGR03965 (HMM): mycofactocin system glycosyltransferaseGO:0051188: cofactor biosynthetic process
GO:0031179: peptide modification
mycofactocin system transcriptional regulatormftRNOTIGR03968 (HMM): mycofactocin system transcriptional regulatorGO:0051193: regulation of cofactor metabolic process
dehydrogenase, SDR familymft_AD1NOTIGR03971 (HMM): SDR family mycofactocin-dependent oxidoreductase
TIGR04504 (HMM): SDR family mycofactocin-dependent oxidoreductase
GO:0055114: oxidation-reduction process
GO:0051188: cofactor biosynthetic process
GO:0031179: peptide modification
dehydrogenase, Rxyl_3153 familymft_AD2YESTIGR03989 (HMM): NDMA-dependent alcohol dehydrogenase, Rxyl_3153 familyGO:0055114: oxidation-reduction process
dehydrogenase, iron-activated familymft_AD3NOPF00465 (HMM-CLUST): alcohol dehydrogenase, iron-containing
TIGR04266 (HMM): NDMA-dependent methanol dehydrogenase
GO:0055114: oxidation-reduction process
GO:0055114: oxidation-reduction process

Parent Properties
GenProp0184cofactor biosynthesis

Sibling Properties
GenProp0001chorismate biosynthesis via shikimate
GenProp0030glutathione biosynthesis
GenProp0036biotin biosynthesis
GenProp0038tetrahydrofolate biosynthesis from GTP and PABA
GenProp0057NAD(P) biosynthesis from L-aspartate and DHAP
GenProp0112riboflavin, FAD and FMN from GTP and ribulose-5-P
GenProp0124pantothenate biosynthesis from aspartate and 2-oxoisovalerate
GenProp0136ubiquinone biosynthesis from chorismate, aerobic
GenProp0140iron-sulfur cluster assembly systems
GenProp0170coenzyme PQQ biosynthesis
GenProp0171coenzyme A biosynthesis from pantothenate
GenProp0194siroheme biosynthesis from uroporphrinogen-III
GenProp0220uroporhyrinogen III biosynthesis from 5-aminolevulinate
GenProp0221protoporphyrin IX biosynthesis from uroporphyrinogen III
GenProp0222protoheme from protoporphyrin IX
GenProp0250thiamine: hydroxyethylthiazole from 1-deoxy-D-xylulose-5-phosphate, cysteine, either Tyr or Gly
GenProp02555-aminolevulinate biosynthesis
GenProp0261thiamin pyrophosphate (TPP) de novo biosynthesis
GenProp0274coenzyme B12 biosynthesis - overview
GenProp0300photosynthetic cofactor biosynthesis
GenProp0466molybdopterin biosynthesis
GenProp0752ergothioneine biosynthesis
GenProp0753mycothiol biosynthesis
GenProp0759para-aminobenzoic acid (PABA) biosynthesis from chorismate
GenProp0791coenzyme F420 biosynthesis
GenProp07927,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) biosynthesis
GenProp0836menaquinone biosynthesis
GenProp0860tryptophan tryptophylquinone modification of methylamine dehydrogenase
GenProp0862pyridoxal phosphate biosynthesis, PdxA/PdxJ pathway
GenProp0863pyridoxal phosphate biosynthesis, Pdx1/Pdx2 pathway
GenProp0891coenzyme M biosynthesis
GenProp0927bacillithiol biosynthesis
GenProp1064ovothiol biosynthesis