Genome Property Definition Page

NameO103(Ec) antigen biosynthesis
DescriptionThe E. coli O103 antigen structure has been determined [1], and the biosynthetic cluster analyzed [2]. The monomer unit is a linear pentasaccharide including one 3-deoxy-3-(R)-3-hydroxybutyramido-D-fucose per unit. The cluster includes four glycosyltransferases (WbtDEFG), but accurate assignment of which enzyme carries out each reaction has not yet been determined. The needed reactions are: 1) aDGalNAc-(1->3)-bDGlcNAc, 2) aDGlcNAc-(1->4)-aDGalNAc, 3) bDFuc3NBu-(1->6)-aDGlcNAc, and 4) bDGlc-(1->2)-bDFuc3NBu.
Parent PropertyGenProp0913: E. coli O-antigen monomer biosynthesis clusters
Literature References
[ 1 ]Vinogradov E, Maclean LL, Perry MB  Structural determination of the O-antigenic polysaccharide of enteropathogenic Escherichia coli O103:H2.  Can J Microbiol. 2010 May;56(5):366-72.  PMID 20555398
[ 2 ]Fratamico PM, DebRoy C, Strobaugh TP Jr, Chen CY  DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes.  Can J Microbiol. 2005 Jun;51(6):515-22.  PMID 16121232

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
source of dTDP-DFuc3NBuFuc3NBuYESGenProp0952 (GENPROP): dTDP-3-deoxy-3-(R)-3-hydroxybutyramino-D-fucose biosynthesis from dTDP-3-aminofucose
O103(Ec)-associated glycosyltransferase WbtDWbtDYES2760 (RULE_BASE)
O103(Ec)-associated glycosyltransferase WbtEWbtEYES2761 (RULE_BASE)
O103(Ec)-associated glycosyltransferase WbtFWbtFYES2763 (RULE_BASE)
O103(Ec)-associated glycosyltransferase WbtGWbtGYES2764 (RULE_BASE)
O103(Ec)-specific transporter (flippase) wzxwzx103YES2759 (RULE_BASE)
O103(Ec)-specific polymerase wzywzy103YES2762 (RULE_BASE)
chain length determinant proteinwzzNOPF02706 (HMM-CLUST): chain length determinant protein

Parent Properties
GenProp0913E. coli O-antigen monomer biosynthesis clusters

Sibling Properties
GenProp0932O104(Ec) antigen biosynthesis
GenProp0947O55(Ec) antigen biosynthesis
GenProp0949O111(Ec) antigen biosynthesis
GenProp0963O2(Ec) antigen biosynthesis
GenProp1014O5(Ec) antigen biosynthesis
GenProp1018O8(Ec) antigen biosynthesis
GenProp1042O128(Ec) antigen biosynthesis