Genome Property Definition Page

Accession

GenProp0963

Name

O2(Ec) antigen biosynthesis

Type

METAPATH

Description

The O2 antigen is a branched pentasaccharide observed in strains 1.2741 and 9.1649. It consists of the repeating unit [->3)a-L-Rha-((2<-1)-a-D-Fuc3NAc)-(1->2)-a-L-Rha-(1->3)-b-L-Rha-(1->4)-b-D-GlcNAc-(1->]. Genes for the biosynthesis of Rhamnose and 3-acylamino-fucose (Fuc3NAc), both of which arise from the same intermediate, 4,6-dideoxy-4-oxo-dTDP-D-glucose, are found within the cluster.

Parent Property

GenProp0913: E. coli O-antigen monomer biosynthesis clusters

Literature References

[ 1 ]Jansson PE, Lennholm H, Lindberg B, Lindquist U, Svenson SB Structural studies of the O-specific side-chains of the Escherichia coli O2 lipopolysaccharide. Carbohydr Res. 1987 Apr 1;161(2):273-9. PMID 3555792
[ 2 ]Fratamico PM, Yan X, Liu Y, DebRoy C, Byrne B, Monaghan A, Fanning S, Bolton D Escherichia coli serogroup O2 and O28ac O-antigen gene cluster sequences and detection of pathogenic E. coli O2 and O28ac by PCR. Can J Microbiol. 2010 Apr;56(4):308-16. PMID 20453897

Components

Step Name	Step Num	Required	Evidence (Method)
source of 3-acetamidofucose	Fuc3NAc	YES	GenProp0964 (GENPROP): dTDP-3-acetamidofucose (dTDP-Fuc3NAc) biosynthesis from dTDP-3-aminofucose
b-L-Rha-(1->4)-b-D-GlcNAc transferase	GTR-1	YES	2770 (RULE_BASE)
a-L-Rha-(1->2)-a-L-Rha, ->3)-b-L-Rha transferase	GTR-2,3	YES	2769 (RULE_BASE)
a-D-Fuc3NAc-(1->2)-a-L-Rha transferase	GTR-4	YES	2768 (RULE_BASE)
source of Rhamnose	Rha	YES	GenProp0183 (GENPROP): dTDP-L-rhamnose biosynthesis from dTDP-4-dehydro-L-rhamnose
regulatory protein GalF	galF	YES	TIGR01105 (HMM): regulatory protein GalF
clustered gnd enzyme	gnd	NO	TIGR00873 (HMM-CLUST): 6-phosphogluconate dehydrogenase (decarboxylating)
O2-antigen specific transporter (flippase)	wzx2	YES	2767 (RULE_BASE)
clustered wzy protein	wzy	YES	2848 (RULE_BASE)
O-antigen specific chain length determinant protein wzz	wzz	YES	2846 (RULE_BASE)

Parent Properties

Accession	Name
GenProp0913	E. coli O-antigen monomer biosynthesis clusters

Sibling Properties

Accession	Name
GenProp0932	O104(Ec) antigen biosynthesis
GenProp0947	O55(Ec) antigen biosynthesis
GenProp0949	O111(Ec) antigen biosynthesis
GenProp0953	O103(Ec) antigen biosynthesis
GenProp1014	O5(Ec) antigen biosynthesis
GenProp1018	O8(Ec) antigen biosynthesis
GenProp1042	O128(Ec) antigen biosynthesis