Genome Property Definition Page

NameO147(Ec) antigen biosynthesis
DescriptionThe E. coli O147 antigen is a linear tetrasaccharide with the structure [->2)aLRha(1->2)aLRha(1->4)bDGalA(1->3)bDGalNAc(1->]. The Shigella flexneri O6 antogen is identical except for an additional 3-O-linked acetyl group on the second rhamnose [1]. Despite requiring three glycosidic linkages in the monomer unit, there are only two glycosyltransferases in the antigen biosynthesis cluster. The WfbY transferase is most closely related (32% identical) to WvwB from the O146 cluster, which is purported to transfer L-rhamnose in two different positions. WfbZ, on the other hand, is most closely related to a series of Gal->GalNAc transferases.
JCVI RoleBiosynthesis and degradation of surface polysaccharides and lipopolysaccharides
Literature References
[ 1 ]Hygge Blakeman K, Weintraub A, Widmalm G  Structural determination of the O-antigenic polysaccharide from the enterotoxigenic Escherichia coli O147.  Eur J Biochem. 1998 Jan 15;251(1-2):534-7.  PMID 9492329
Web ReferencesECODAB:O147

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
source of L-rhamnoseL-RhaYESGenProp0183 (GENPROP): dTDP-L-rhamnose biosynthesis from dTDP-4-dehydro-L-rhamnose
alpha-L-rhamnosyltransferase WfbYWfbYYES2925 (RULE_BASE)
N-acetyl-D-galactose beta-1,3-D-galctosuronyltransferase WfbZWfbZYES2926 (RULE_BASE)
O147(Ec)-antigen specific transporter (flippase) wzxwzx147YES2924 (RULE_BASE)
O147(Ec)-antigen specific polymerase wzywzy147YES2923 (RULE_BASE)