HMM Summary Page: TIGR00048

Function23S rRNA (adenine(2503)-C(2))-methyltransferase
Gene SymbolrlmN
Trusted Cutoff290.00
Domain Trusted Cutoff290.00
Noise Cutoff285.00
Domain Noise Cutoff285.00
Isology Typeequivalog
EC Number2.1.1.192
HMM Length355
Mainrole CategoryUnknown function
Subrole CategoryEnzymes of unknown specificity
AuthorRichardson DL, Haft DH
Entry DateApr 20 1999 2:04PM
Last ModifiedFeb 14 2011 3:27PM
CommentMembers of this family are RlmN, a 23S rRNA m2A2503 methyltransferase in the radical SAM enzyme family. Closely related is Cfr, a Staphylococcus sciuri plasmid-borne homolog to this family, Cfr, has been identified as essential to transferrable resistance to chloramphenicol and florfenicol. Cfr methylates 23S RNA at a different site.
ReferencesDR PFAM; PF04055; Radical SAM superfamily RN [1] RM PMID:18025251 RT The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA. RA Toh SM, Xiong L, Bae T, Mankin AS RL RNA. 2008 Jan;14(1):98-106. Epub 2007 Nov 19. RN [2] RM 10952608 RT Identification of a plasmid-borne chloramphenicol-florfenicol resistance gene in Staphylococcus sciuri. RA Schwarz S, Werckenthin C, Kehrenberg C. RL Antimicrob Agents Chemother 2000 Sep;44(9):2530-3 RN [3] RM 11222759 RT Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods. RA Sofia HJ, Chen G, Hetzler BG, Reyes-Spindola JF, Miller NE. RL Nucleic Acids Res 2001 Mar 1;29(5):1097-106 DR HAMAP; MF_01849; 521 of 570