| Accession | TIGR00169 |
| Name | leuB |
| Function | 3-isopropylmalate dehydrogenase |
| Gene Symbol | leuB |
| Trusted Cutoff | 353.20 |
| Domain Trusted Cutoff | 353.20 |
| Noise Cutoff | 253.75 |
| Domain Noise Cutoff | 253.75 |
| Isology Type | equivalog |
| EC Number | 1.1.1.85 |
| HMM Length | 349 |
| Mainrole Category | Amino acid biosynthesis |
| Subrole Category | Pyruvate family |
| Gene Ontology Term | GO:0003862: 3-isopropylmalate dehydrogenase activity molecular_function |
| | GO:0009098: leucine biosynthetic process biological_process |
| Author | Haft DH |
| Entry Date | Apr 20 1999 2:08PM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.
Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.
This HMM will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase.
|
| References | DR PROSITE; PDOC00389;
RM 97250497
RT Protein engineering reveals ancient adaptive replacements in isocitrate dehydrogenase.
SE TIGR
GA hmmls
AL clustalw, belvu, seaview
DR HAMAP; MF_01033; 218 of 219 |
| Genome Property | GenProp0164: leucine biosynthesis from pyruvate and acetyl-CoA (HMM) |
