|Domain Trusted Cutoff||150.10|
|Domain Noise Cutoff||127.00|
|Mainrole Category||Purines, pyrimidines, nucleosides, and nucleotides|
|Subrole Category||Purine ribonucleotide biosynthesis|
|Gene Ontology Term||GO:0004644: phosphoribosylglycinamide formyltransferase activity molecular_function|
| ||GO:0009152: purine ribonucleotide biosynthetic process biological_process|
|Entry Date||Oct 25 1999 11:40AM|
|Last Modified||Feb 14 2011 3:27PM|
|Comment||This HMM describes phosphoribosylglycinamide formyltransferase (GAR transformylase), one of several proteins in formyl_transf (Pfam family PF00551). This enzyme uses formyl tetrahydrofolate as a formyl group donor to produce 5'-phosphoribosyl-N-formylglycinamide. PurT, a different GAR transformylase, uses ATP and formate rather than formyl tetrahydrofolate. Experimental proof includes complementation of E. coli purN mutants by orthologs from vertebrates (where it is a domain of a multifunctional protein), Bacillus subtilis, and Arabidopsis.
No archaeal example was detected.
In phylogenetic analyses, the member from Saccharomyces cerevisiae shows a long branch length but membership in the family, while the formyltetrahydrofolate deformylases form a closely related outgroup.|
DR PFAM; PF00551; formyl_transf
DR YPD; ADE8
DR OUTGROUP; EGAD|15350|EC1232
DR OUTGROUP; EGAD|156449|AA1257
RT Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12.
RA Smith JM, Daum HA 3d
RL J Biol Chem 1987 Aug 5;262(22):10565-9|
|Genome Property||GenProp0110: purine (inosine-5'-phosphate) biosynthesis from ribose-5-phosphate (HMM)|
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