HMM Summary Page: TIGR01422

Functionphosphonoacetaldehyde hydrolase
Gene SymbolphnX
Trusted Cutoff221.20
Domain Trusted Cutoff221.20
Noise Cutoff117.40
Domain Noise Cutoff117.40
Isology Typeequivalog
EC Number3.11.1.1
HMM Length253
Mainrole CategoryCentral intermediary metabolism
Subrole CategoryOther
Gene Ontology TermGO:0009636: response to toxin biological_process
GO:0016827: hydrolase activity, acting on acid carbon-phosphorus bonds molecular_function
GO:0019635: 2-aminoethylphosphonate catabolic process biological_process
GO:0019636: phosphonoacetate metabolic process biological_process
GO:0019700: organic phosphonate catabolic process biological_process
AuthorSelengut J
Entry DateJan 28 2002 10:59AM
Last ModifiedFeb 14 2011 3:27PM
CommentThis enzyme catalyzes the cleavage of the carbon phosphorous bond of a phosphonate. The mechanism depends on the substrate having a carbonyl one carbon away from the cleavage position. This enzyme is a member of the Haloacid Dehalogenase (HAD) superfamily of aspartate-nucleophile hydrolases (PF00702), and contains a modified version of the conserved catalytic motifs of that superfamily: the first motif is usually DxDx(T/V), here it is DxAxT, and in the third motif the normal conserved lysine is instead an arginine. Additionally, the enzyme contains a unique conserved catalytic lysine (B. cereus pos. 53) which is involved in the binding and activation of the substrate through the formation of a Schiff base. The substrate of this enzyme is the product of 2-aminoethylphosphonate (AEP) transaminase, phosphonoacetaldehyde. This degradation pathway for AEP may be related to its toxic properties which are utilized by microorganisms as a chemical warfare agent.
ReferencesRN [1] RM 10956028 RT The crystal structure of bacillus cereus phosphonoacetaldehyde hydrolase: insight into catalysis of phosphorus bond cleavage and catalytic diversification within the HAD enzyme superfamily. RA Morais MC, Zhang W, Baker AS, Zhang G, Dunaway-Mariano D, Allen KN RL Biochemistry 2000 Aug 29;39(34):10385-96. DR PFAM; PF00702 DR EXPERIMENTAL; GP|10835405|pdb|1FEZ|A; Bacillus cereus DR OUTGROUP; EGAD|155362|AA0933; Aquifex aolicus; phosphoglycolate phosphatase (pgp) DR HAMAP; MF_01375; 42 of 42
Genome PropertyGenProp0238: 2-aminoethylphosphonate catabolism to acetaldehyde (HMM)