HMM Summary Page: TIGR01660

Functionnitrate reductase, beta subunit
Gene SymbolnarH
Trusted Cutoff524.05
Domain Trusted Cutoff524.05
Noise Cutoff348.00
Domain Noise Cutoff348.00
Isology Typeequivalog
EC Number1.7.99.4
HMM Length492
Mainrole CategoryEnergy metabolism
Subrole CategoryAnaerobic
Gene Ontology TermGO:0008940: nitrate reductase activity molecular_function
GO:0009061: anaerobic respiration biological_process
GO:0009325: nitrate reductase complex cellular_component
AuthorDaugherty S
Entry DateAug 30 2002 4:53PM
Last ModifiedFeb 14 2011 3:27PM
CommentThe Nitrate reductase enzyme complex allows bacteria to use nitrate as an electron acceptor during anaerobic growth. The enzyme complex consists of a tetramer that has an alpha, beta and 2 gamma subunits. The alpha and beta subunits have catalytic activity and the gamma subunits attach the enzyme to the membrane and is a b-type cytochrome that receives electrons from the quinone pool and transfers them to the beta subunit. This model is specific for the beta subunit for nitrate reductase I (narH) and nitrate reductase II (narY) for gram positive and gram negative bacteria.A few thermophiles and archaea also match the model.The seed members used in this model are all experimentally characterized and include the following:SP:P11349, and SP:P19318, both E.Coli (NarH and NarY respectively), SP:P42176 from B. Subtilis, GP:11344602 from Psuedomonas fluorescens,GP:541762 from Paracoccus denitrificans, and GP:18413622 from Halomonas halodenitrificans. This HMM also matches PFAM PF00037 for 4Fe-4S binding domain.
ReferencesRN [1] RM PMID:2233673 RT Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon RA Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M RL Mol Gen Genet 1990 Jun;222(1):104-11 RN [2] RM PMID:2674654 RT Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer. RA Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V. RL Mol Gen Genet 1989 Aug;218(2):249-56
Genome PropertyGenProp0636: respiratory nitrate reductase (HMM)