HMM Summary Page: TIGR01700
Accession | TIGR01700 |
Name | PNPH |
Function | purine nucleoside phosphorylase I, inosine and guanosine-specific |
Trusted Cutoff | 293.35 |
Domain Trusted Cutoff | 293.35 |
Noise Cutoff | 288.70 |
Domain Noise Cutoff | 288.70 |
Isology Type | equivalog |
EC Number | 2.4.2.1 |
HMM Length | 249 |
Mainrole Category | Purines, pyrimidines, nucleosides, and nucleotides |
Subrole Category | Salvage of nucleosides and nucleotides |
Gene Ontology Term | GO:0004731: purine-nucleoside phosphorylase activity molecular_function |
GO:0006139: nucleobase-containing compound metabolic process biological_process | |
Author | Selengut J |
Entry Date | Sep 24 2002 10:02PM |
Last Modified | Feb 14 2011 3:27PM |
Comment | This model represents a family of bacterial and metazoan purine phosphorylases acting primarily on inosine and guanosine and not acting on adenosine. PNP-I refers to the nomenclature from Bacillus stearothermophilus [1] where PHP-II refers to the nucleotidase acting on adenosine as the primary substrate. The bacterial enzymes (PUNA) are typified by the Bacilus PupG protein [2] which is involved in the metabolism of nucleosides as a carbon source. Several metazoan enzymes (PNPH) are well characterized including the human [3] and bovine [4] enzymes which have been crystallized. |
References | RN [1] RM PMID: 9058965 RT Cloning and expression of purine nucleoside phosphorylase I gene from Bacillus stearothermophilus TH 6-2. RA Hamamoto T, Okuyama K, Noguchi T, Midorikawa Y. RL Biosci Biotechnol Biochem. 1997 Feb;61(2):272-5. RN [2] RM PMID: 10537218 RT Nucleosides as a carbon source in Bacillus subtilis: characterization of the drm-pupG operon. RA Schuch R, Garibian A, Saxild HH, Piggot PJ, Nygaard P. RL Microbiology 1999 Oct;145 ( Pt 10):2957-66 RN [3] RM PMID: 2104852 RT Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2 A resolution. RA Ealick SE, Rule SA, Carter DC, Greenhough TJ, Babu YS, Cook WJ, Habash J, Helliwell JR, Stoeckler JD, Parks RE Jr, et al. RL J Biol Chem. 1990 Jan 25;265(3):1812-20. RN [4] RM PMID: 9020983 RT Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with hypoxanthine at 2.15 A resolution. RA Koellner G, Luic M, Shugar D, Saenger W, Bzowska A. RL J Mol Biol. 1997 Jan 17;265(2):202-16. |