|Function||periplasmic nitrate reductase, large subunit|
|Domain Trusted Cutoff||930.55|
|Domain Noise Cutoff||472.50|
|Mainrole Category||Energy metabolism|
|Gene Ontology Term||GO:0008940: nitrate reductase activity molecular_function|
| ||GO:0009325: nitrate reductase complex cellular_component|
| ||GO:0042126: nitrate metabolic process biological_process|
| ||GO:0055114: oxidation-reduction process biological_process|
|Entry Date||Sep 26 2002 11:05AM|
|Last Modified||Feb 14 2011 3:27PM|
|Comment||This model represents the large subunit of a family of nitrate reductases found in proteobacteria which are localized to the periplasm. This subunit binds molybdopterin and contains a twin-arginine motif at the N-terminus. The protein associates with NapB, a soluble heme-containing protein and NapC, a membrane-bound cytochrome c.
The periplasmic nitrate reductases are not involved in the assimilation of nitrogen, and are not directly involved in the formation of electrochemical gradients (i.e. respiration) either. Rather, the purpose of this enzyme is either dissimilatory (i.e. to dispose of excess reductive equivalents) or indirectly respiratory by virtue of the consumption of electrons derived from NADH via the proton translocating NADH dehydrogenase .
The enzymes from Alicagenes eutrophus  and Paracoccus pantotrophus  have been characterized. In E. coli  (as well as other organisms) this gene is part of a large nitrate reduction operon (napFDAGHBC).|
RM PMID: 8376334
RT Structure and function of a periplasmic nitrate reductase in Alcaligenes eutrophus H16
RA RA Siddiqui, U Warnecke-Eberz, A Hengsberger, B Schneider, S Kostka and B Friedrich
RL J. Bacteriol., 09 1993, 5867-5876, Vol 175, No. 18
RM PMID: 7639719
RT The napEDABC gene cluster encoding the periplasmic nitrate reductase system of Thiosphaera pantotropha.
RA Berks B.C., Richardson D.J., Reilly A., Willis A.C., Ferguson S.J.;
RL Biochem. J. 309:983-992(1995).
RM PMID: 11844760
RT Periplasmic nitrate reductase (NapABC enzyme) supports anaerobic respiration by Escherichia coli K-12.
RA Stewart V, Lu Y, Darwin AJ.
RL J Bacteriol 2002 Mar;184(5):1314-23
RM PMID: 9720883
RT Definition and distinction between assimilatory, dissimilatory and respiratory pathways.
RA Moreno-Vivian C, Ferguson SJ.
RL Mol Microbiol 1998 Jul;29(2):664-6
DR HAMAP; MF_01630; 89 of 93|
|Genome Property||GenProp0200: periplasmic nitrate reductase (HMM)|
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