|Function||sulfite reductase (NADPH) hemoprotein, beta-component|
|Domain Trusted Cutoff||756.55|
|Domain Noise Cutoff||707.50|
|Mainrole Category||Central intermediary metabolism|
|Subrole Category||Sulfur metabolism|
|Gene Ontology Term||GO:0000103: sulfate assimilation biological_process|
| ||GO:0004783: sulfite reductase (NADPH) activity molecular_function|
|Entry Date||Oct 28 2003 3:23PM|
|Last Modified||Feb 14 2011 3:27PM|
|Comment||Sulfite reductase (NADPH) catalyzes a six electron reduction of sulfite to sulfide in prokaryotic organisms. It is a complex oligomeric enzyme composed of two different peptides with a subunit composition of alpha(8)-beta(4) . The alpha component, encoded by cysJ, is a flavoprotein containing both FMN and FAD, while the beta component, encoded by cysI, is a siroheme, iron-sulfur protein .
In Salmonella typhimurium and Escherichia coli, both the alpha and beta subunits of sulfite reductase are located in a unidirectional gene cluster along with phosphoadenosine phosphosulfate reductase, which catalyzes a two step reduction of PAPS to give free sulfite .
In cyanobacteria and plant species, sulfite reductase ferredoxin (EC 126.96.36.199) catalyzes the reduction of sulfite to sulfide .|
RT Sulfite reductase structure at 1.6 A: evolution and catalysis for reduction of inorganic anions.
RA Crane BR, Siegel LM, Getzoff ED.
RL Science. 1995 Oct 6;270(5233):59-67.
RT Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase.
RA Ostrowski J, Wu JY, Rueger DC, Miller BE, Siegel LM, Kredich NM.
RL J Biol Chem. 1989 Sep 15;264(26):15726-37.
RT The ferredoxin:sulphite reductase gene from Synechococcus PCC7942.
RA Gisselmann G, Klausmeier P, Schwenn JD.
RL Biochim Biophys Acta. 1993 Aug 16;1144(1):102-6.
DR PFAM; PF01077; Nitrite and sulphite reductase 4Fe-4S domain
DR PFAM; PF03460; Nitrite/Sulfite reductase ferredoxin-like half domain
DR PROSITE; PS00365; Nitrite and sulfite reductases iron-sulfur/siroheme-binding site.
DR EXPERIMENTAL; SP|P17846; Escherichia coli; isolated as a homodimer exhibiting phospho-adenylylsulphate reductase (PAPS reductase) activity
DR OUTGROUP; GP|1113082; Plectonema boryanum; nitrite reductase
DR HAMAP; MF_01540; 71 of 72|
|Genome Property||GenProp0149: sulfate reduction to sulfide, assimilatory (HMM)|
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