HMM Summary Page: TIGR02155

Functionphenylacetate-CoA ligase
Gene SymbolpaaF
Trusted Cutoff646.30
Domain Trusted Cutoff646.30
Noise Cutoff597.50
Domain Noise Cutoff597.50
Isology Typeequivalog
EC Number6.2.1.30
HMM Length422
Mainrole CategoryEnergy metabolism
Subrole CategoryOther
Gene Ontology TermGO:0010124: phenylacetate catabolic process biological_process
GO:0047475: phenylacetate-CoA ligase activity molecular_function
AuthorBrinkac L
Entry DateApr 2 2004 3:11PM
Last ModifiedFeb 14 2011 3:27PM
CommentPhenylacetate-CoA ligase (PA-CoA ligase) catalyzes the first step in aromatic catabolism of phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA) [1]. Often located in a conserved gene cluster with enzymes involved in phenylacetic acid activation (paaG/H/I/J), phenylacetate-CoA ligase has been found among the proteobacteria as well as in gram positive prokaryotes [2]. In the B-subclass proteobacterium Azoarcus evansii, phenylacetate-CoA ligase has been shown to be induced under aerobic and anaerobic growth conditions. It remains unclear however, whether this induction is due to the same enzyme or to another isoenzyme restricted to specific anaerobic growth conditions [3].
ReferencesRN [1] RM PMID: 9748275 RT Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway. RA Ferrandez A, Minambres B, Garcia B, Olivera ER, Luengo JM, Garcia JL, Diaz E. RL J Biol Chem. 1998 Oct 2;273(40):25974-86. RN [2] RM PMID: 11260461 RT The phenylacetyl-CoA catabolon: a complex catabolic unit with broad biotechnological applications. RA Luengo JM, Garcia JL, Olivera ER. RL Mol Microbiol. 2001 Mar;39(6):1434-42. RN [3] RM PMID: 10629172 RT Biochemical and molecular characterization of phenylacetate-coenzyme A ligase, an enzyme catalyzing the first step in aerobic metabolism of phenylacetic acid in Azoarcus evansii. RA El-Said Mohamed M. RL J Bacteriol. 2000 Jan;182(2):286-94. DR PFAM; PF00501; AMP-binding protein DR EXPERIMENTAL; SP|P76085; Escherichia coli; specific activity dependent on the presence of ATP, CoA, and PA; in vivo formation of PA-CoA; mutagenesis. DR EXPERIMENTAL; GP|6715111; Azoarcus evansii; specific activity measured in PA-grown cells; in vivo formation of PA-CoA; molecular and catalytic properties determined. DR OUTGROUP; OMNI|NTL01MT01493; Methanobacterium thermoautotrophicum; coenzyme F390 synthetase I
Genome PropertyGenProp0210: phenylacetic acid catabolism (HMM)