HMM Summary Page: TIGR02493

Functionpyruvate formate-lyase 1-activating enzyme
Gene SymbolpflA
Trusted Cutoff231.75
Domain Trusted Cutoff231.75
Noise Cutoff203.45
Domain Noise Cutoff203.45
Isology Typeequivalog
EC Number1.97.1.4
HMM Length235
Mainrole CategoryEnergy metabolism
Subrole CategoryAnaerobic
Gene Ontology TermGO:0018307: enzyme active site formation biological_process
GO:0043365: [formate-C-acetyltransferase]-activating enzyme activity molecular_function
AuthorSelengut J
Entry DateFeb 25 2005 3:34PM
Last ModifiedFeb 14 2011 3:27PM
CommentAn iron-sulfur protein with a radical-SAM domain (PF04055). A single glycine residue in EC, formate C-acetyltransferase (formate-pyruvate lyase), is oxidized to the corresponding radical by transfer of H from its CH2 to AdoMet with concomitant cleavage of the latter. The reaction requires Fe2+. The first stage is reduction of the AdoMet to give methionine and the 5'-deoxyadenosin-5-yl radical, which then abstracts a hydrogen radical from the glycine residue [1].
ReferencesRN [1] RM PMID: 8175649 RT Adenosylmethionine-dependent synthesis of the glycyl radical in pyruvate formate-lyase by abstraction of the glycine C-2 pro-S hydrogen atom. Studies of [2H]glycine-substituted enzyme and peptides homologous to the glycine 734 site. RA Frey M, Rothe M, Wagner AF, Knappe J. RL J Biol Chem. 1994 Apr 29;269(17):12432-7.
Genome PropertyGenProp0943: pyruvate formate-lyase system (HMM)