HMM Summary Page: TIGR03297

Functionphosphonopyruvate decarboxylase
Gene SymbolaepY
Trusted Cutoff312.50
Domain Trusted Cutoff312.50
Noise Cutoff237.85
Domain Noise Cutoff237.85
Isology Typeequivalog
EC Number4.1.1.82
HMM Length360
Gene Ontology TermGO:0017000: antibiotic biosynthetic process biological_process
GO:0033980: phosphonopyruvate decarboxylase activity molecular_function
AuthorSelengut J
Entry DateJan 31 2007 11:33AM
Last ModifiedFeb 14 2011 3:27PM
CommentThis family consists of examples of phosphonopyruvate an decarboxylase enzyme that produces phosphonoacetaldehyde (Pald), the second step in the biosynthesis phosphonate-containing compounds. Since the preceding enzymate step, PEP phosphomutase (AepX, TIGR02320) favors the substrate PEP energetically, the decarboxylase is required to drive the reaction in the direction of phosphonate production. Pald is a precursor of natural products including antibiotics like bialaphos [1] and phosphonothricin [2] in Streptomyces species, phosphonate-modified molecules such as the polysaccharide B of Bacteroides fragilis [3], the phosphonolipids of Tetrahymena pyroformis [4], the glycosylinositolphospholipids of Trypanosoma cruzi [5]. This gene generally occurs in prokaryotic organisms adjacent to the gene for AepX. Most often an aminotansferase (aepZ) is also present which leads to the production of the most common phosphonate compound, 2-aminoethylphosphonate (AEP).
ReferencesRN [1] RM PMID: 1337066 RT Studies on the biosynthesis of bialaphos (SF-1293). 14. Nucleotide sequence of phosphoenolpyruvate phosphomutase gene isolated from a bialaphos producing organism, Streptomyces hygroscopicus, and its expression in Streptomyces lividans. RA Hidaka T, Hidaka M, Seto H RL J Antibiot (Tokyo). 1992 Dec;45(12):1977-80. RN [2] RM PMID: 9673017 RT Isolation and characterization of the PEP-phosphomutase and the phosphonopyruvate decarboxylase genes from the phosphinothricin tripeptide producer Streptomyces viridochromogenes Tu494. RA Schwartz D, Recktenwald J, Pelzer S, Wohlleben W RL FEMS Microbiol Lett. 1998 Jun 15;163(2):149-57. RN [3] RM PMID: 11035722 RT Bacteroides fragilis NCTC9343 produces at least three distinct capsular polysaccharides: cloning, characterization, and reassignment of polysaccharide B and C biosynthesis loci. RA Coyne MJ, Kalka-Moll W, Tzianabos AO, Kasper DL, Comstock LE RL Infect Immun. 2000 Nov;68(11):6176-81. RN [4] RM PMID: 1547241 RT Phosphonate biosynthesis: molecular cloning of the gene for phosphoenolpyruvate mutase from Tetrahymena pyriformis and overexpression of the gene product in Escherichia coli. RA Seidel HM, Pompliano DL, Knowles JR RL Biochemistry. 1992 Mar 10;31(9):2598-608. RN [5] RM PMID: 12672809 RT Properties of phosphoenolpyruvate mutase, the first enzyme in the aminoethylphosphonate biosynthetic pathway in Trypanosoma cruzi. RA Sarkar M, Hamilton CJ, Fairlamb AH RL J Biol Chem. 2003 Jun 20;278(25):22703-8. Epub 2003 Apr 2.
Genome PropertyGenProp0724: phosphonoacetaldehyde biosynthesis from phosphoenolpyruvate (HMM)