|Function||pyrrolysine--tRNA ligase, N-terminal region|
|Domain Trusted Cutoff||51.15|
|Domain Noise Cutoff||50.40|
|Entry Date||Jan 5 2010 5:19PM|
|Last Modified||Feb 14 2011 3:27PM|
|Comment||PylS is the enzyme responsible for charging the pyrrolysine tRNA, PylT, by ligating a free molecule of pyrrolysine. Pyrrolysine is encoded at an in-frame UAG (amber) at least in several corrinoid-dependent methyltransferases of the archaeal genera Methanosarcina and Methanococcoides, such as trimethylamine methyltransferase. This protein occurs as a fusion protein in Methanosarcina but as split genes in Desulfitobacterium hafniense and other bacteria. This model describes the small, N-terminal region.|
RT Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.
RA Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA.
RL Nature. 2004 Sep 16;431(7006):333-5.
RT An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.
RA Polycarpo C, Ambrogelly A, Berube A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Soll D.
RL Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12450-4.
DR HAMAP; MF_01573; 6 of 6|
|Genome Property||GenProp0312: pyrrolysine (HMM)|
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