HMM Summary Page: TIGR04403

Functionsporulation killing factor system radical SAM maturase
Gene SymbolskfB
Trusted Cutoff300.00
Domain Trusted Cutoff300.00
Noise Cutoff200.00
Domain Noise Cutoff200.00
Isology Typeequivalog
HMM Length402
AuthorHaft DH
Entry DateJan 26 2013 5:26PM
Last ModifiedJan 26 2013 5:26PM
CommentMembers of this family are a radical SAM enzyme of post-translational modification of ribosomally translated peptides. In Bacillus subtilis, the enzyme SkfB creates a sactipeptide (sulfur-to-alpha-carbon) crosslink of Cys-4 to Met-12 of the mature form of sporulation killing factor (SkfA). In Paenibacillus larvae subsp. larvae B-3650, the Met is replaced by Leu, so the modification must be different. SkfB has 2 4Fe-4S clusters, one in its radical SAM domain (PF04055) and one in a region that somewhat resembles the SPASM domain (TIGR04085).
ReferencesRN [1] RM PMID:23282011 RT Two [4Fe-4S] Clusters Containing Radical SAM Enzyme SkfB Catalyze Thioether Bond Formation during the Maturation of the Sporulation Killing Factor. RA Fluhe L, Burghaus O, Wieckowski BM, Giessen TW, Linne U, Marahiel MA RL J Am Chem Soc. 2013 Jan 23;135(3):959-62. doi: 10.1021/ja310542g.
Genome PropertyGenProp1078: sporulation killing factor system (HMM)