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About

Biographies

Daniel Gibson, Ph.D.
Associate Professor

Research Interests and Accomplishments

Daniel Gibson is an Associate Professor in the Synthetic Biology group at the JCVI. Since joining the JCVI as a postdoc in 2004, Gibson led the JCVI efforts to synthesize two complete bacterial genomes. Those projects resulted in creation of the first synthetic bacterial cell and development of an enabling suite of DNA synthesis and assembly methods.

Prior to joining the JCVI, Gibson earned his Ph.D. in Molecular Biology from the University of Southern California. While there, he used yeast as the model system for studying cell cycle surveillance mechanisms (checkpoints), which are significant in our understanding of cancer development. Before earning his Ph.D., he earned his Bachelor's degree in Biological Sciences from the State University of New York at Buffalo.

Select Publications

Gibson, D. G.
Gene and Genome Construction In Yeast

Curr Protoc Mol Biol. 2011 Apr 01; 0: Unit3 22.[more]

Noskov, V. N., Chuang, R. Y., et al.
Isolation of Circular Yeast Artificial Chromosomes for Synthetic Biology and Functional Genomics Studies

Nat Protoc. 2011 Jan 01; 6(1): 89-96.[more]

Gibson, D. G., Smith, H. O., et al.
Chemical Synthesis of the Mouse Mitochondrial Genome

Nat Methods. 2010 Oct 10;[more]

Gibson, D. G., Glass, J. I., et al.
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

Science. 2010 Jul 02; 329(5987): 52-6.[more]

Benders, G. A., Noskov, V. N., et al.
Cloning Whole Bacterial Genomes In Yeast

Nucleic Acids Res. 2010 Mar 07; 38(8): 2558-69.[more]

Gibson, D. G.
Synthesis of DNA Fragments In Yeast by One-step Assembly of Overlapping Oligonucleotides

Nucleic Acids Res. 2009 Nov 01; 37(20): 6984-90.[more]

Lartigue, C., Vashee, S., et al.
Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered In Yeast

Science. 2009 Aug 20;[more]

Gibson, D. G., Young, L., et al.
Enzymatic Assembly of DNA Molecules Up to Several Hundred Kilobases

Nat Methods. 2009 Apr 12; 6(5): 343-345.[more]

Gibson, D. G., Benders, G. A., et al.
One-step Assembly In Yeast of 25 Overlapping DNA Fragments to Form a Complete Synthetic Mycoplasma genitalium Genome

Proc Natl Acad Sci U S A. 2008 Dec 23; 105(51): 20404-9.[more]

Gibson, D. G., Benders, G. A., et al.
Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

Science. 2008 Jan 24; 319(5867): 1215-20.[more]