About
Biographies
Research Interests and Accomplishments
Ray-Yuan Chuang is an Assistant Professor in the Synthetic Biology Group at the JCVI. Dr. Chuang has extensive experience in yeast genetic, biochemistry and molecular biology. He has studied the mechanism of initiation of DNA replication in yeast Schizosaccharomyces pombe and initiation of mRNA translation in yeast Saccharomyces cerevisiae. Dr. Chuang has developed several yeast genetic tools specific for engineering bacterial genome cloned in yeast. He is also a co-investor of a technique, called Tandem Repeat-Endonuclease Cleavage, for seamless modifications of bacterial genomes cloned in yeast.
Dr. Chuang received his Ph.D. in 1997 from the Ohio State University, and did postdoctoral work at Johns Hopkins University, School of Medicine.
Select Publications
Weyman, P. D., Vargas, W. A., et al.
Heterologous Expression of Alteromonas macleodii and Thiocapsa Roseopersicina [NiFe] Hydrogenases In Escherichia coli
Microbiology. 2011 Feb 24;[more]
Noskov, V. N., Chuang, R. Y., et al.
Isolation of Circular Yeast Artificial Chromosomes for Synthetic Biology and Functional Genomics Studies
Nat Protoc. 2011 Jan 01; 6(1): 89-96.[more]
Gibson, D. G., Glass, J. I., et al.
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Science. 2010 Jul 02; 329(5987): 52-6.[more]
Noskov, V. N., Segall-Shapiro, T. H., et al.
Tandem Repeat Coupled With Endonuclease Cleavage (TREC): a Seamless Modification Tool for Genome Engineering In Yeast
Nucleic Acids Res. 2010 May 08; 38(8): 2570-6.[more]
Benders, G. A., Noskov, V. N., et al.
Cloning Whole Bacterial Genomes In Yeast
Nucleic Acids Res. 2010 Mar 07; 38(8): 2558-69.[more]
Montague, M., Barnes, C., et al.
The Evolution of RecD Outside of the RecBCD Complex
J Mol Evol. 2009 Oct 20;[more]
Lartigue, C., Vashee, S., et al.
Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered In Yeast
Science. 2009 Aug 20;[more]
Gibson, D. G., Young, L., et al.
Enzymatic Assembly of DNA Molecules Up to Several Hundred Kilobases
Nat Methods. 2009 Apr 12; 6(5): 343-345.[more]