JCVI: About / Bios / Roger Lasken
 
 
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About

Biographies

Roger Lasken, Ph.D.
Director of Single Cell Genomics
Professor

Research Interests and Accomplishments

Roger Lasken has spent much of his career studying DNA polymerases, the enzymes that synthesize DNA in the cell.  Postdoctoral work was with Arthur Kornberg, Nobel Laureate, and discoverer of these enzymes.  Dr. Lasken was Assistant Professor at Cornell University Medical College and Principal Scientist at Life Technologies Inc where DNA polymerase was used in biotechnology applications to amplify DNA in the lab.  As Director of Genomics at Molecular Staging Inc, he developed Multiple Displacement Amplification (MDA), a method to amplify DNA that is sold under the brand names Repli-g (Qiagen) and TempliPhi and GenomiPhi (GE Healthcare) and is used in hundreds of laboratories.  Dr. Lasken's team was the first to sequence genomic DNA from a single cell. In 2004 he returned to academics as Professor at The Center for Genomic Sciences, Allegheny General Hospital, Pittsburgh where single cell genomics was applied to the study of infectious disease.  He joined the J. Craig Venter Institute in 2006 and has focused on integrating his technologies into the scientific programs of the Institute.  Projects include discovery of environmental microbes, genomics of human pathogens, and genetics of cancer cells.

Select Publications

McConnell MJ, Lindberg MR, et al.
Mosaic Copy Number Variation in Human Neurons.

Science (New York, N.Y.). 2013 Nov 01; 342: 632-7.[more]

Ishoey, T., Woyke, T., et al.
Genomic Sequencing of Single Microbial Cells from Environmental Samples

Curr Opin Microbiol. 2008 Jun 10; 11(3): 198-204.[more]

Lasken, R. S.
Single-cell Genomic Sequencing Using Multiple Displacement Amplification

Curr Opin Microbiol. 2007 Oct 01; 10(5): 510-6.[more]

Lasken, R. S., Stockwell, T. B.
Mechanism of Chimera Formation During the Multiple Displacement Amplification Reaction

BMC Biotechnol. 2007 Apr 12; 7: 19.[more]

Raghunathan, A., Ferguson, H. R., Jr., et al.
Genomic DNA Amplification from a Single Bacterium

Appl Environ Microbiol. 2005 Jun 01; 71(6): 3342-7.[more]

Hosono, S., Faruqi, A. F., et al.
Unbiased Whole-genome Amplification Directly from Clinical Samples

Genome Res. 2003 May 01; 13(5): 954-64.[more]

Dean, F. B., Hosono, S., et al.
Comprehensive Human Genome Amplification Using Multiple Displacement Amplification

Proc Natl Acad Sci U S A. 2002 Apr 16; 99(8): 5261-6.[more]

Dean, F. B., Nelson, J. R., et al.
Rapid Amplification of Plasmid and Phage DNA Using Phi 29 DNA Polymerase and Multiply-primed Rolling Circle Amplification

Genome Res. 2001 Jun 01; 11(6): 1095-9.[more]