JCVI: About / Bios / Rembert Pieper
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Rembert Pieper, Ph.D.
Associate Professor

Research Interests and Accomplishments 

Dr. Pieper has been an Associate Professor at the J. Craig Venter Institute (Rockville site) since 2009. He was also Associate Director of the NIAID-funded Pathogen Functional Genomics Resource Center, at the J. Craig Venter Institute from 2006-2011 and at The Institute for Genomic Research from 2003-2006. His laboratory is primarily an analytical biochemistry laboratory, with technology emphasis on proteomics and protein expression. Dr. Pieper and his research group are currently involved in several projects integrating proteomics as a key discipline into system biology. There is a particular interest to understand cell biological processes perturbed or modulated in infectious diseases (host-pathogen interactions), in autoimmune diseases, and in synthetically modified / genetically engineered microbial organisms. Some of these projects have a practical side, including the discovery of new biomarkers (Type I diabetes, urinary tract infection) and the characterization of subcellular vaccine candidates. The laboratory also collaborates with JCVI's genomics and computational biologists to advance microbiome research, a particularly challenging field of research in systems biology. 

Dr. Pieper obtained his Ph.D. degree from the Institute of Applied Chemistry, Technical University of Berlin, Germany (1989-1993). He conducted post-doctoral research in the Department of Chemical Engineering, Stanford University (1994-1996), and the National Cancer Institute (1997-1998). He was a Staff Scientist and Director for Protein Chemistry at Large Scale Biology Corporation (1999-2003), with an emphasis on protein biomarker discovery for non-communicable diseases. He has co-authored 12 patent applications (eight of which have been issued) and 40 original research or review articles.


U.S. Patent #7,342,089 (issued 1/5/10): Immunoaffinity-Based Subtraction Method

U.S. Patent # 6,710,189 B2 (issued 3/24/04): Cell-Free Synthesis of Polyketides

U.S. Patent # 7,101,684 (issued 2/27/04): Modified Modular Polyketide Synthase

U.S. Patent # 6,274,560 (issued 8/14/01): Cell-Free Synthesis of Polyketides

U.S. Patent # 6,080,555 (issued 6/27/00): Synthesis of Polyketides from Diketides

U.S. Patent # 6,066,721 (issued 5/23/00): Method to Produce Novel Polyketides

Select Publications

Suh MJ, Fedorova ND, et al.
Development Stage-specific Proteomic Profiling Uncovers Small, Lineage Specific Proteins Most Abundant In the Aspergillus fumigatus Conidial Proteome.

Proteome science. 2012 Apr 30; 10: 30.[more]

Pieper, R., Zhang, Q., et al.
Characterizing the Escherichia coli O157:H7 Proteome Including Protein Associations With Higher Order Assemblies

PLoS ONE. 2011 Nov 30; 6(11): e26554.[more]

Kuntumalla S, Zhang Q, et al.
In Vivo Versus In Vitro Protein Abundance Analysis of Shigella dysenteriae Type 1 Reveals Changes In the Expression of Proteins Involved In Virulence, Stress and Energy Metabolism.

BMC microbiology. 2011 Jun 01; 11: 147.[more]

Payne, S. H., Huang, S. T., et al.
A Proteogenomic Update to Yersinia: Enhancing Genome Annotation

BMC Genomics. 2010 Aug 05; 11(1): 460.[more]

Pieper, R., Huang, S. T., et al.
Proteomic Analysis of Iron Acquisition, Metabolic and Regulatory Responses of Yersinia pestis to Iron Starvation

BMC Microbiol. 2010 Jan 29; 10(1): 30.[more]

Lartigue, C., Glass, J. I., et al.
Genome Transplantation In Bacteria: Changing One Species to Another

Science. 2007 Aug 03; 317(5838): 632-8.[more]

Pieper, R., Gatlin, C. L., et al.
Characterization of the Human Urinary Proteome: a Method for High-resolution Display of Urinary Proteins on Two-dimensional Electrophoresis Gels With a Yield of Nearly 1400 Distinct Protein Spots

Proteomics. 2004 Apr 01; 4(4): 1159-74.[more]

Pieper, R., Gatlin, C. L., et al.
The Human Serum Proteome: Display of Nearly 3700 Chromatographically Separated Protein Spots on Two-dimensional Electrophoresis Gels and Identification of 325 Distinct Proteins

Proteomics. 2003 Jul 01; 3(7): 1345-64.[more]

Pieper R, Su Q, et al.
Multi-component Immunoaffinity Subtraction Chromatography: an Innovative Step Towards a Comprehensive Survey of the Human Plasma Proteome.

Proteomics. 2003 Apr 01; 3: 422-32.[more]