JCVI: About / Bios / Yo Suzuki
 
 
Section Banner

About

Biographies

Yo Suzuki, Ph.D.
Assistant Professor

Research Interests and Accomplishments

Yo Suzuki is an Assistant Professor in the Synthetic Biology and Bioenergy Department at the JCVI. He uses the 'Green Monster' method for genome engineering. In this method, GFP-marked genomic alterations such as deletions and insertions are assembled into a single genome via repeated rounds of mating, meiosis, and flow-cytometric enrichment of yeast cells harboring the alterations. He is interested in (1) generating yeast multi-mutants to reveal previously unidentified genetic interactions, (2) introducing exogenous biosynthetic pathways into yeast to make valuable compounds, and (3) engineering bacterial genomes residing in yeast to help characterize the minimal cellular life.

Yo earned his Ph.D. in molecular biology from the University of Colorado for his work on a C. elegans TGF-b gene with Bill Wood. While in Colorado, he became interested in the problem of multi-gene genetic interaction. As a postdoctoral fellow with Min Han, he identified genes that act in parallel with a C. elegans homolog of the tumor suppressor gene PTEN. He switched to yeast and developed the Green Monster method for multi-locus engineering in Fritz Roth's lab at Harvard Medical School. He joined the JCVI in 2010.

Select Publications

Karas BJ, Jablanovic J, et al.
Direct Transfer of Whole Genomes from Bacteria to Yeast.

Nature Methods. 2013 May 01; 10: 410-2.[more]

Suzuki Y, Stam J, et al.
The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions.

Journal of Visualized Experiments : JoVE. 2012 Mar 01;: e4072.[more]

Cokol M, Chua HN, et al.
Systematic Exploration of Synergistic Drug Pairs.

Molecular systems biology. 2011 Apr 01; 7: 544.[more]

Suk K, Choi J, et al.
Reconstitution of Human RNA Interference In Budding Yeast.

Nucleic Acids Research. 2011 Apr 01; 39: e43.[more]

Suzuki, Y., St Onge, R. P., et al.
Knocking Out Multigene Redundancies Via Cycles of Sexual Assortment and Fluorescence Selection

Nat Methods. 2011 Feb 01; 8(2): 159-64.[more]

Suzuki, Y., Han, M.
Genetic Redundancy Masks Diverse Functions of the Tumor Suppressor Gene PTEN During C. elegans Development

Genes Dev. 2006 Feb 15; 20(4): 423-8.[more]

Suzuki, Y., Roth, F. P.
Systematic Genetics Swims Forward Elegantly

Mol Syst Biol. 2006 Jan 01; 2: 48.[more]

Suzuki Y, Morris GA, et al.
A Cuticle Collagen Encoded by the Lon-3 Gene May Be a Target of TGF-beta Signaling In Determining Caenorhabditis Elegans Body Shape.

Genetics. 2002 Dec 01; 162: 1631-9.[more]

Suzuki, Y., Yandell, M. D., et al.
A BMP Homolog Acts as a Dose-dependent Regulator of Body Size and Male Tail Patterning In Caenorhabditis elegans

Development. 1999 Jan 01; 126(2): 241-50.[more]