Goldberg, S. M., Johnson, J., Busam, D., Feldblyum, T., Ferriera, S., Friedman, R., Halpern, A., Khouri, H., Kravitz, S. A., Lauro, F. M., Li, K., Rogers, Y. H., Strausberg, R., Sutton, G., Tallon, L., Thomas, T., Venter, E., Frazier, M., Venter, J. C.
A Sanger/pyrosequencing Hybrid Approach for the Generation of High-quality Draft Assemblies of Marine Microbial Genomes
Proc Natl Acad Sci U S A. 2006 Jul 13; 103(30): 11240-5.
Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.