JCVI: Chemical Synthesis of the Mouse Mitochondrial Genome
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Gibson, D. G., Smith, H. O., Hutchison, C. A., 3rd, Venter, J. C., Merryman, C.

Chemical Synthesis of the Mouse Mitochondrial Genome

Nat Methods. 2010 Oct 10;

PubMed Citation


We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.