JCVI: Cloning and Characterization of a Gene Cluster for Geldanamycin Production In Streptomyces Hygroscopicus NRRL 3602
 
 
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Citation

Rascher, A., Hu, Z., Viswanathan, N., Schirmer, A., Reid, R., Nierman, W. C., Lewis, M., Hutchinson, C. R.

Cloning and Characterization of a Gene Cluster for Geldanamycin Production In Streptomyces Hygroscopicus NRRL 3602

FEMS Microbiol Lett. 2003 Jan 28; 218(2): 223-30.

PubMed Citation

Abstract

We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602.