Qian QM, Wang YS, Yang HX, Xue JH, Liu L, Zhou B, Wang JC, Yin JC
Colorimetric Detection of Metallothioneins Using a Thymine-rich Oligonucleotide-Hg Complex and Gold Nanoparticles.
Analytical Biochemistry. 2013 Jan 25;
A simple and sensitive method for label-free, colorimetric detection of metallothioneins (MTs) has been developed by using a thymine (T)-rich oligonucleotide (TRO)-Hg-AuNPs system. In this colorimetric strategy, the thiol groups of MTs could interact with mercury from T-Hg(2+)-T complex to release TRO, resulting in a color change of the system. The response signals linearly correlated with the concentration of MTs over the ranges of 2.56 × 10(-8) - 3.08 × 10(-7) mol L(-1), and the limit of detection (LOD) is 7.67 × 10(-9) mol L(-1). The relative standard deviation and the recovery were 2.3% - 4.8% (n =11) and 94.2 - 103.9%, respectively. The proposed method avoids the label and derivatization steps in common methods, and allows direct analysis of the samples by the naked eyes without costly instruments, and was reliable, inexpensive and sensitive.