Read, T. D., Salzberg, S. L., Pop, M., Shumway, M., Umayam, L., Jiang, L., Holtzapple, E., Busch, J. D., Smith, K. L., Schupp, J. M., Solomon, D., Keim, P., Fraser, C. M.
Comparative Genome Sequencing for Discovery of Novel Polymorphisms In Bacillus anthracis
Science. 2002 Jun 14; 296(5575): 2028-33.
Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (SNPs), inserted or deleted sequences, and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences among different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.