JCVI: Entamoeba histolytica Lectins Contain Unique 6-Cys or 8-Cys Chitin-binding Domains
 
 
Section Banner

Publications

Citation

Van Dellen, K., Ghosh, S. K., Robbins, P. W., Loftus, B., Samuelson, J.

Entamoeba histolytica Lectins Contain Unique 6-Cys or 8-Cys Chitin-binding Domains

Infect Immun. 2002 Jun 01; 70(6): 3259-63.

PubMed Citation

Abstract

The Jacob lectin, the most abundant glycoprotein in the cyst wall of Entamoeba invadens, contains five unique 6-Cys chitin-binding domains (CBDs). We identified Entamoeba histolytica and Entamoeba dispar genes encoding Jacob homologues, each of which contains two predicted 6-Cys CBDs. A unique 8-Cys CBD was found at the N termini of the E. histolytica chitinase and three other predicted lectins, called Jessie 1 to Jessie 3. The CBDs of four E. histolytica lectins (Jacob, chitinase, and Jessies 2 and 3) were expressed in secretory vesicles of transfected amebae and shown to bind to particulate chitin.