Knight, M., Miller, A. N., Geoghagen, N. S. M., Lewis, F. A., Kerlavage, A. R.
Expressed Sequence Tags (ESTs) of Biomphalaria Glabrata, an Intermediate Snail Host of Schistosoma Mansoni: Use In the Identification of RFLP Markers
Malacologia. 1998 Jan 01; 39(1): 175-182.
To identify some of the genes expressed in the snail Biomphalaria glabrata, a major intermediate host for the trematode parasite Schistosoma mansoni, we sequenced random cDNA clones from either a whole body or a cerebral ganglia cDNA library to generate 111 expressed sequence tags (ESTs). Searches of existing public databases showed that the majority of the snail sequences (54%) showed no significant homology to genes listed in either DNA or protein databases. Thirty one EST sequences showed significant matches with other genes in the databases. These included genes involved in gene expression, such as ribosomal proteins and translation factors, and those involved in cell communication, such as acetylcholine receptor and ATP-dependent transporter. Some ESTs used as probes demonstrated the occurrence of restriction fragment length polymorphisms (RFLPs) between parasite-resistant and parasite-susceptible snail stocks. Southern hybridization of parasite DNA with the snail EST encoding the acetylcholine receptor as probe showed the presence of a related sequence in the parasite genome, with the heterologous probe, indicating that this may be a useful method to identify closely related genes between the host and parasite.