JCVI: Functional and Taxonomic Dynamics of an Electricity-consuming Methane-producing Microbial Community.
 
 
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Citation

Bretschger O, Carpenter K, Phan T, Suzuki S, Ishii S, Grossi-Soyster E, Flynn M, Hogan J

Functional and Taxonomic Dynamics of an Electricity-consuming Methane-producing Microbial Community.

Bioresource Technology. 2015 Nov 01; 195. : 254-64.

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Abstract

The functional and taxonomic microbial dynamics of duplicate electricity-consuming methanogenic communities were observed over a 6 months period to characterize the reproducibility, stability and recovery of electromethanogenic consortia. The highest rate of methanogenesis was 0.72 mg-CH4/L/day, which occurred during the third month of enrichment when multiple methanogenic phylotypes and associated Desulfovibrionaceae phylotypes were present in the electrode-associated microbial community. Results also suggest that electromethanogenic microbial communities are very sensitive to electron donor-limiting open-circuit conditions. A 45 min exposure to open-circuit conditions induced an 87% drop in volumetric methane production rates. Methanogenic performance recovered after 4 months to a maximum value of 0.30 mg-CH4/L/day under set potential operation (-700 mV vs Ag/AgCl); however, current consumption and biomass production was variable over time. Long-term functional and taxonomic analyses from experimental replicates provide new knowledge toward understanding how to enrich electromethanogenic communities and operate bioelectrochemical systems for stable and reproducible performance.