JCVI: Gene and Genome Construction In Yeast
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Gibson, D. G.

Gene and Genome Construction In Yeast

Curr Protoc Mol Biol. 2011 Apr 01; 0: Unit3 22.

PubMed Citation


The yeast Saccharomyces cerevisiae has the capacity to take up and assemble dozens of different overlapping DNA molecules in one transformation event. These DNA molecules can be single-stranded oligonucleotides, to produce gene-sized fragments, or double-stranded DNA fragments, to produce molecules up to hundreds of kilobases in length, including complete bacterial genomes. This unit presents protocols for designing the DNA molecules to be assembled, transforming them into yeast, and confirming their assembly. Curr. Protoc. Mol. Biol. 94:3.22.1-3.22.17. (c) 2011 by John Wiley & Sons, Inc.