Publications
Citation
Sabri, M., Hauser, R., Ouellette, M., Liu, J., Dehbi, M., Moeck, G., Garcia, E., Titz, B., Uetz, P., Moineau, S.
Genome annotation and intra-viral interactome of the Streptococcus pneumoniae virulent phage Dp-1
J Bacteriol. 2010 Nov 19;
Abstract
Streptococcus pneumoniae causes several diseases including pneumonia, septicaemia, and meningitis. Phage Dp-1 is one of the very few isolated virulent S. pneumoniae bacteriophages, but only a partial characterization is currently available. Here, we confirmed that Dp-1 belongs to the Siphoviridae family. Then, we determined its complete genomic sequence of 56,506 bp. It encodes 72 open reading frames of which 44 have been assigned a function. We have identified putative promoters, Rho-independent terminators and several genomic clusters. We provide evidence that Dp-1 may be using a novel DNA replication system as well as redirecting host protein synthesis through queuosine-containing tRNAs. Liquid chromatography-mass spectrometry analysis of purified phage Dp-1 particles identified at least eight structural proteins. Finally, using comprehensive Yeast Two-Hybrid screens we identified 156 phage protein interactions and this intra-viral interactome was used to propose a structural model of Dp-1.