JCVI: Genome Annotation and Intra-viral Interactome of the Streptococcus pneumoniae Virulent Phage Dp-1
 
 
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Sabri, M., Hauser, R., Ouellette, M., Liu, J., Dehbi, M., Moeck, G., Garcia, E., Titz, B., Uetz, P., Moineau, S.

Genome Annotation and Intra-viral Interactome of the Streptococcus pneumoniae Virulent Phage Dp-1

J Bacteriol. 2010 Nov 19;

PubMed Citation

Abstract

Streptococcus pneumoniae causes several diseases including pneumonia, septicaemia, and meningitis. Phage Dp-1 is one of the very few isolated virulent S. pneumoniae bacteriophages, but only a partial characterization is currently available. Here, we confirmed that Dp-1 belongs to the Siphoviridae family. Then, we determined its complete genomic sequence of 56,506 bp. It encodes 72 open reading frames of which 44 have been assigned a function. We have identified putative promoters, Rho-independent terminators and several genomic clusters. We provide evidence that Dp-1 may be using a novel DNA replication system as well as redirecting host protein synthesis through queuosine-containing tRNAs. Liquid chromatography-mass spectrometry analysis of purified phage Dp-1 particles identified at least eight structural proteins. Finally, using comprehensive Yeast Two-Hybrid screens we identified 156 phage protein interactions and this intra-viral interactome was used to propose a structural model of Dp-1.