JCVI: Genomic DNA Amplification from a Single Bacterium
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Raghunathan, A., Ferguson, H. R., Jr., Bornarth, C. J., Song, W., Driscoll, M., Lasken, R. S.

Genomic DNA Amplification from a Single Bacterium

Appl Environ Microbiol. 2005 Jun 01; 71(6). : 3342-7.

PubMed Citation


Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.

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