JCVI: Grape-seed Derived Procyanidins Interfere With Adipogenesis of 3T3-L1 Cells at the Onset of Differentiation
 
 
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Pinent, M., Blade, M. C., Salvado, M. J., Arola, L., Hackl, H., Quackenbush, J., Trajanoski, Z., Ardevol, A.

Grape-seed Derived Procyanidins Interfere With Adipogenesis of 3T3-L1 Cells at the Onset of Differentiation

Int J Obes Relat Metab Disord. 2005 May 10; 29(8): 934-41.

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Abstract

OBJECTIVE: Our group's previous results on the effects of a grape seed procyanidin extract (GSPE) on adipose metabolism showed that peroxisome proliferator-activated receptor-gamma (PPARgamma) plays a central role in the lipolytic effects of GSPE on adipocytes. Since PPARgamma2 is a main regulator of the differentiation process of adipocytes, we investigated whether GSPE affects the adipogenesis of 3T3-L1 cells.DESIGN: We performed a time point screening by treating 3T3-L1 cells with GSPE during the differentiation process for 24 h.MEASUREMENTS: Differentiation markers and differential gene expression due to GSPE treatment (using the microarray technique).RESULTS: Twenty four hour-GSPE treatment at the onset of differentiation reduces adipose-specific markers and maintains the expression of preadipocyte marker preadipocyte factor-1 (Pref-1) significantly elevated. These effects were not found in other time points. Microarray analysis of gene expression after GSPE treatment at the early stage of differentiation showed a modified gene expression profile in which cell cycle and growth-related genes were downregulated by GSPE.CONCLUSION: These results suggest that GSPE affects adipogenesis, mainly at the induction of differentiation, and that procyanidins may have a new role in which they impede the formation of adipose cells.International Journal of Obesity advance online publication, 10 May 2005; doi:10.1038/sj.ijo.0802988.