JCVI: Improved Method for Selecting RNA-binding Activities In Vivo
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Fouts, D. E., Celander, D. W.

Improved Method for Selecting RNA-binding Activities In Vivo

Nucleic Acids Res. 1996 Apr 15; 24(8): 1582-4.

PubMed Citation


RNA challenge phages are modified versions of bacteriophage P22 that allow one to select directly for a specific RNA-protein interaction in vivo. The original construction method for generating a bacteriophage that encodes a specific RNA target requires two homologous recombination reactions between plasmids and phages in bacteria. An improved method is described that enables one to readily construct RNA challenge phages through a single homologous recombination reaction in vivo. We have applied the new method to construct a derivative of P22R17, an RNA challenge phage that undergoes lysogenic development in bacterial cells that express the bacteriophage R17/MS2 coat protein.

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