In Vivo Versus In Vitro Protein Abundance Analysis of Shigella dysenteriae Type 1 Reveals Changes In the Expression of Proteins Involved In Virulence, Stress and Energy Metabolism.
BMC microbiology. 2011 Jun 01; 11: 147.
Shigella dysenteriae serotype 1 (SD1) causes the most severe form of epidemic bacillary dysentery. Quantitative proteome profiling of Shigella dysenteriae serotype 1 (SD1) in vitro (derived from LB cell cultures) and in vivo (derived from gnotobiotic piglets) was performed by 2D-LC-MS/MS and APEX, a label-free computationally modified spectral counting methodology.