JCVI: Infection and Genotype Remodel the Entire Soybean Transcriptome.
 
 
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Citation

Zhou L, Mideros SX, Bao L, Hanlon R, Arredondo FD, Tripathy S, Krampis K, Jerauld A, Evans C, St Martin SK, Maroof MA, Hoeschele I, Dorrance AE, Tyler BM

Infection and Genotype Remodel the Entire Soybean Transcriptome.

BMC genomics. 2009 May 01; 10: 49.

External Citation

Abstract

High throughput methods, such as high density oligonucleotide microarray measurements of mRNA levels, are popular and critical to genome scale analysis and systems biology. However understanding the results of these analyses and in particular understanding the very wide range of levels of transcriptional changes observed is still a significant challenge. Many researchers still use an arbitrary cut off such as two-fold in order to identify changes that may be biologically significant. We have used a very large-scale microarray experiment involving 72 biological replicates to analyze the response of soybean plants to infection by the pathogen Phytophthora sojae and to analyze transcriptional modulation as a result of genotypic variation.

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