JCVI: Opinion: Facilitating Genome Navigation: Survey Sequencing and Dense Radiation-hybrid Gene Mapping
 
 
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Hitte, C., Madeoy, J., Kirkness, E. F., Priat, C., Lorentzen, T. D., Senger, F., Thomas, D., Derrien, T., Ramirez, C., Scott, C., Evanno, G., Pullar, B., Cadieu, E., Oza, V., Lourgant, K., Jaffe, D. B., Tacher, S., Dreano, S., Berkova, N., Andre, C., Deloukas, P., Fraser, C., Lindblad-Toh, K., Ostrander, E. A., Galibert, F.

Opinion: Facilitating Genome Navigation: Survey Sequencing and Dense Radiation-hybrid Gene Mapping

Nat Rev Genet. 2005 Jul 12; 6(8): 643-8.

PubMed Citation

Abstract

Accurate and comprehensive sequence coverage for large genomes has been restricted to only a few species of specific interest. Lower sequence coverage (survey sequencing) of related species can yield a wealth of information about gene content and putative regulatory elements. But survey sequences lack long-range continuity and provide only a fragmented view of a genome. Here we show the usefulness of combining survey sequencing with dense radiation-hybrid (RH) maps for extracting maximum comparative genome information from model organisms. Based on results from the canine system, we propose that from now on all low-pass sequencing projects should be accompanied by a dense, gene-based RH map-construction effort to extract maximum information from the genome with a marginal extra cost.