JCVI: Proteomics and Metaproteomics
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Pieper R, Huang ST, Suh MJ

Proteomics and Metaproteomics

Encyclopedia of Metagenomics (SPi Global). 2014 Apr 08;: 1-11.


Proteomics pertains to the comprehensive analysis of expressed proteins from a cell, a multicellular system, an extracellular environment, or a large set of recombinant clones. This is achieved using combinations of protein separation, identification, and/or assay techniques, such as liquid chromatography-mass spectrometry (LC-MS), two-dimensional gel electrophoresis-mass spectrometry (2DE-MS), affinity purification-mass spectrometry (AP-MS), and protein- or antibody-based microarrays. The objectives in proteomics research can be diverse; they include protein quantification on a global scale, highly parallel analysis of protein functions and interactions, structural characterization of protein complexes, unraveling trafficking of proteins and their distribution in different cellular compartments, and discovery of protein signatures for a disease state or other perturbation.