JCVI: Recombinase-mediated Cassette Exchange (RMCE) System for Functional Genomics Studies in Mycoplasma Mycoides.
 
 
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Citation

Noskov VN, Ma L, Chen S, Chuang RY

Recombinase-mediated Cassette Exchange (RMCE) System for Functional Genomics Studies in Mycoplasma Mycoides.

Biological Procedures Online. 2015 Jul 01; 17: 6.

External Citation

Abstract

We have previously established technologies enabling us to engineer the Mycoplasma mycoides genome while cloned in the yeast Saccharomyces cerevisiae, followed by genome transplantation into Mycoplasma capricolum recipient cells to produce M. mycoides with an altered genome. To expand the toolbox for genomic modifications, we designed a strategy based on the Cre/loxP-based Recombinase-Mediated Cassette Exchange (RMCE) system for functional genomics analyses.