Hansen, A., Chen, Y., Inman, J. M., Phan, Q. N., Qi, Z. Q., Xiang, C. C., Palkovits, M., Cherman, N., Kuznetsov, S. A., Robey, P. G., Mezey, E., Brownstein, M. J.
Sensitive and Specific Method for Detecting G Protein-coupled Receptor MRNAs
Nat Methods. 2007 Jan 01; 4(1): 35-7.
G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.