Rexroad, C. E., 3rd, Lee, Y., Keele, J. W., Karamycheva, S., Brown, G., Koop, B., Gahr, S. A., Palti, Y., Quackenbush, J.
Sequence Analysis of a Rainbow Trout CDNA Library and Creation of a Gene Index
Cytogenet Genome Res. 2003 Jan 01; 102(1): 347-54.
Expressed sequence tag (EST) projects have produced extremely valuable resources for identifying genes affecting phenotypes of interest. A large-scale EST sequencing project for rainbow trout was initiated to identify and functionally annotate as many unique transcripts as possible. Over 45,000 5' ESTs were obtained by sequencing clones from a single normalized library constructed using mRNA from six tissues. The production of this sequence data and creation of a rainbow trout Gene Index eliminating redundancy and providing annotation for these sequences will facilitate research in this species.