JCVI: Sequencing Viral Genomes from a Single Isolated Plaque.
 
 
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Citation

Depew J, Zhou B, McCorrison JM, Wentworth DE, Purushe J, Koroleva G, Fouts DE

Sequencing Viral Genomes from a Single Isolated Plaque.

Virology Journal. 2013 Jun 06; 10: 181.

External Citation

Abstract

Whole genome sequencing of viruses and bacteriophages is often hindered because of the need for large quantities of genomic material. A method is described that combines single plaque sequencing with an optimization of Sequence Independent Single Primer Amplification (SISPA). This method can be used for de novo whole genome next-generation sequencing of any cultivable virus without the need for large-scale production of viral stocks or viral purification using centrifugal techniques.