JCVI: Streptomyces turgidiscabies Car8 Contains a Modular Pathogenicity Island That Shares Virulence Genes With Other Actinobacterial Plant Pathogens
 
 
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Huguet-Tapia, J. C., Badger, J. H., Loria, R., Pettis, G. S.

Streptomyces turgidiscabies Car8 Contains a Modular Pathogenicity Island That Shares Virulence Genes With Other Actinobacterial Plant Pathogens

Plasmid. 2010 Nov 15;

PubMed Citation

Abstract

Streptomyces turgidiscabies Car8 is an actinobacterium that causes the economically important disease potato scab. Pathogenesis in this species is associated with a mobile pathogenicity island (PAISt) that site specifically inserts into the bacA gene in Streptomyces spp. Here we provide the 674,223 bp sequence of PAISt, which consists of two non-overlapping modules of 105,364 bp and 568,859 bp. These modules are delimited by three copies of an 8 bp palindromic sequence (TTCATGAA), that also is the integration site (att) of the element. Putative tyrosine recombinase (IntSt) and excisionase (XisSt) proteins are encoded just upstream of att-R. PAISt has regions of synteny to pathogenic, symbiotic and saprophytic actinomycetes. The 105,364 bp PAISt module is identical to a genomic island in Streptomyces scabies 87-22, while the 568,859 bp module contains only a short region of synteny to that genome. However, both modules contain previously characterized and candidate virulence genes.